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PMID: 2615763 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Common nodABC genes in Nod locus 1 of Azorhizobium caulinodans: nucleotide sequence and plant-inducible expression.

Molecular & general genetics : MGG ·Vol. 219 ·No. 1-2 ·1989-10-00 ·Pages 289-98

Goethals K, Gao M, Tomekpe K, Van Montagu M, Holsters M

Abstract

Azorhizobium caulinodans strain ORS571 induces nitrogen-fixing nodules on roots and stem-located root primordia of Sesbania rostrata. Two essential Nod loci have been previously identified in the bacterial genome, one of which (Nod locus 1) shows weak homology with the common nodC gene of Rhizobium meliloti. Here we present the nucleotide sequence of this region and show that it contains three contiguous open reading frames (ORFA, ORFB and ORFC) that are related to the nodABC genes of Rhizobium and Bradyrhizobium species. ORFC is followed by a fourth (ORF4) and probably a fifth (ORF5) open reading frame. ORF4 may be analogous to the nodI gene of R. leguminosarum, whereas ORF5 could be similar to the rhizobial nodF genes. Coordinated expression of this set of five genes seems likely from the sequence organization. There is no typical nod promoter consensus sequence (nod box) in the region upstream of the first gene (ORFA) and there is no nodD-like gene. LacZ fusions constructed with ORFA, ORFB, ORFC, and ORF4 showed inducible beta-galactosidase expression in the presence of S. rostrata seedlings as well as around stem-located root primordia. Among a series of phenolic compounds tested, the flavanone naringenin was the most efficient inducer of the expression of this ORS571 nod gene cluster.

MeSH Terms
Amino Acid Sequence Amino Acids/analysis Bacterial Proteins/analysis,genetics Base Sequence Gene Expression Regulation, Bacterial Genes, Bacterial Molecular Sequence Data Multigene Family Plants/genetics Regulatory Sequences, Nucleic Acid Restriction Mapping Rhizobiaceae/genetics Sequence Homology, Nucleic Acid
Chemicals
Amino Acids Bacterial Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Goethals K
Laboratorium voor Genetica, Rijksuniversiteit Gent, Belgium.
Gao M
Tomekpe K
Van Montagu M
Holsters M
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30 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00261190
Published
1989-10-00
Pages
289-98
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00261190
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