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PMID: 1093171 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Fusion of the Escherichia coli lac genes to the ara promoter: a general technique using bacteriophage Mu-1 insertions.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 3 ·1975-03-00 ·Pages 809-13

Casadaban MJ

Abstract

The lac genes were fused to the ara promoter by means of phage phi 80 translocations of the lac and ara genes to att80. Homology for a crossover between the nonhomologous ara and lac operons was provided by mu insertions. Selection for recombinants within the mu insertions generated strains that had the ara promoter on one side of a mu insertion and the lac genes on the other side. ara-lac fusions were obtained from these strains by deleting the mu insertion. These fusions extend the techniques available for studies on the lac operon to studies on the ara operon. It should be possible to fuse other operons by this method.

MeSH Terms

Arabinose/metabolism Chromosome Mapping Coliphages Crosses, Genetic Escherichia coli/metabolism Genes Genetic Techniques Genotype Lactose/metabolism Lysogeny Operon Recombination, Genetic Transduction, Genetic

Chemicals

Arabinose Lactose

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Casadaban M J

References (15)

15 references, click to expand

Prophage deletion mapping of bacteriophage Mu-1.
Virology. 1973 Jul;54(1):93-101 PMID: 4351616
Linkage map of Escherichia coli strain K-12.
Bacteriol Rev. 1972 Dec;36(4):504-24 PMID: 4568762
Escherichia coli K-12 F-prime factors, old and new.
Bacteriol Rev. 1972 Dec;36(4):587-607 PMID: 4568765
Inhibition of lacZ gene translation initiation in trp-lac fusion strains.
J Bacteriol. 1974 Mar;117(3):1231-9 PMID: 4591949
The promoter-operator region of the lac operon of Escherichia coli.
J Mol Biol. 1968 Dec;38(3):413-20 PMID: 4887877
Arabinose-leucine deletion mutants of Escherichia coli B-r.
J Bacteriol. 1969 Jun;98(3):1159-69 PMID: 4892369
A mechanism for repressor action.
J Mol Biol. 1969 Jul 14;43(1):201-13 PMID: 4897791
L-Arabinose-sensitive, L-ribulose 5-phosphate 4-epimerase-deficient mutants of Escherichia coli.
J Bacteriol. 1962 Jul;84:137-46 PMID: 13890280
Detection and isolation of the repressor protein for the tryptophan operon of Escherichia coli.
Proc Natl Acad Sci U S A. 1972 May;69(5):1100-3 PMID: 4338582
Isolating tryptophan regulatory mutants in Escherichia coli by using a trp-lac fusion strain.
J Bacteriol. 1972 Feb;109(2):526-32 PMID: 4550812
Genetic mapping of prophage Mu.
Virology. 1973 Jul;54(1):109-16 PMID: 4576741
Frameshift mutations in the lactose operon of E. coli.
Cold Spring Harb Symp Quant Biol. 1966;31:189-201 PMID: 4866375
The internal low-efficiency promoter of the tryptophan operon of Escherichia coli.
J Mol Biol. 1968 Dec;38(3):447-51 PMID: 4887879
Directed transposition of the arabinose operon: a technique for the isolation of specialized transducing bacteriophages for any Escherichia coli gene.
J Mol Biol. 1969 Aug 28;44(1):117-27 PMID: 4897798
Fusions of the lac and trp Regions of the Escherichia coli Chromosome.
J Bacteriol. 1970 Dec;104(3):1273-9 PMID: 16559103

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.72.3.809

Published

1975-03-00

Pages

809-13

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC432409

Subset

External Links

PubMed source DOI: 10.1073/pnas.72.3.809 PMC Full Text View journal details

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Fusion of the Escherichia coli lac genes to the ara promoter: a general technique using bacteriophage Mu-1 insertions.

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