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PMID: 16593994 Published · ppublish English Journal Article

Rhizobium meliloti nodA and nodB genes are involved in generating compounds that stimulate mitosis of plant cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 22 ·1988-11-00 ·Pages 8578-82

Schmidt J, Wingender R, John M, Wieneke U, Schell J

Abstract

The nodB gene of Rhizobium meliloti encodes a 23.8-kDa protein that is conserved in several Rhizobium species. Monospecific polyclonal antibodies against NodB were used to localize this protein in the cytosol of R. meliloti and Escherichia coli cells containing nodABC genes. In comparison to the NodA and NodC proteins, NodB is synthesized in a disproportionately low amount. The NodA and NodB proteins are involved in generating small, heat-stable compounds that stimulate the mitosis of various plant protoplasts. Our experiments suggest that NodC is not involved in the synthesis of the factors. On the basis of their properties, we speculate that the factors are cytokinin-like substances.

Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Schmidt J
Max-Planck-Institut für Züchtungsforschung, Abt. Schell, D-5000 Cologne 30, Federal Republic of Germany.
Wingender R
John M
Wieneke U
Schell J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.22.8578
Published
1988-11-00
Pages
8578-82
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282502
External Links
PubMed source DOI: 10.1073/pnas.85.22.8578 PMC Full Text View journal details
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