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PMID: 2562992 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Regulation of poly(A) site selection in adenovirus.

Journal of virology ·Vol. 63 ·No. 2 ·1989-02-00 ·Pages 532-41

Falck-Pedersen E, Logan J

Abstract

We have investigated the mechanisms involved in the early-to-late RNA-processing switch which regulates the mRNA species generated from the adenovirus major late transcription unit (MLTU). In particular, polyadenylation choice mechanisms were characterized by using a reconstructed adenovirus E1A gene as a site for insertion of MLTU poly(A) regulation signals (L1 and L3). Adenovirus constructs containing the variant poly(A) recognition elements were used to compare E1A poly(A) signal utilization with wild-type MLTU (L1 to L5) utilization. In both early and late stages of infection, either polyadenylation site (L1 or L3) is capable of being utilized when presented as the only operational poly(A) site. In an early infection, a virus which contains multiple elements presented in tandem (L13) uses the first poly(A) site, L1, preferentially (ratio of L1 to L3, 8:1) in both E1A and MLTU loci. Transcription termination is not involved in restricting the utilization of the downstream L3 site. In a late infection, when each of the five MLTU poly(A) sites is used, a switch also occurs for the E1AL13 construct, with utilization of both the L1 and L3 poly(A) sites. The switch from early to late was not the result of altered processing factors in the late infection, as demonstrated by superinfecting the E1AL13 construct into cells which had already entered a late stage of infection. The superinfecting virus gave an L1-only phenotype; therefore, a cis mechanism is involved in adenovirus poly(A) regulation.

MeSH Terms
Adenoviridae/genetics Adenovirus Early Proteins Genes, Regulator Genes, Switch Genes, Synthetic Genes, Viral Oncogene Proteins, Viral/genetics Poly A/metabolism RNA Processing, Post-Transcriptional RNA, Messenger/metabolism RNA, Viral/metabolism Transcription, Genetic
Chemicals
Adenovirus Early Proteins Oncogene Proteins, Viral RNA, Messenger RNA, Viral Poly A
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Falck-Pedersen E
Hearst Research Foundation, Department of Microbiology, Cornell University Medical College, New York, New York 10021.
Logan J
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.2.532-541.1989
Published
1989-02-00
Pages
532-41
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC247721
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.63.2.532-541.1989 PMC Full Text View journal details
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