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PMID: 2962857 Published · ppublish English Journal Article

Activation of adenovirus 5 E1A transcription by region E1B in transformed primary rat cells.

The EMBO journal ·Vol. 6 ·No. 11 ·1987-11-00 ·Pages 3399-405

Jochemsen AG, Peltenburg LT, te Pas MF, de Wit CM, Bos JL, van der Eb AJ

Abstract

The human adenovirus 5 E1A region can immortalize primary cultures of baby rat kidney cells, but requires the presence of the E1B region for complete oncogenic transformation. One of the effects of the E1B region in the transformation process is the activation of E1A expression. We have investigated the mechanism of this stimulation of E1A expression using nuclear run-on assays with nuclei from Ad5 E1A- and Ad5 E1-transformed cells. It was found that E1B enhances E1A at the level of transcription-initiation. This activation is mainly observed when the E1A and E1B regions are integrated simultaneously into the cellular genome and only minimally when these genes are integrated separately, strongly suggesting that a close physical linkage of these regions is essential for the observed effect.

MeSH Terms
Adenovirus Early Proteins Adenoviruses, Human/genetics Animals Cell Line Cell Transformation, Viral Genes Genes, Viral Oncogene Proteins, Viral/genetics Plasmids RNA, Messenger/genetics Transcription, Genetic Transfection
Chemicals
Adenovirus Early Proteins Oncogene Proteins, Viral RNA, Messenger
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Jochemsen A G
Department of Medical Biochemistry, Sylvius Laboratories, University of Leiden, The Netherlands.
Peltenburg L T
te Pas M F
de Wit C M
Bos J L
van der Eb A J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-11-00
Pages
3399-405
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553797
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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