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PMID: 2560166 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

An electrophysiological investigation of the characteristics and function of GABAA receptors on bovine adrenomedullary chromaffin cells.

Pflugers Archiv : European journal of physiology ·Vol. 415 ·No. 1 ·1989-10-00 ·Pages 95-103

Peters JA, Lambert JJ, Cottrell GA

Abstract

The characteristics and function of gamma-aminobutyric acidA (GABAA) receptors expressed on bovine chromaffin cells in culture have been investigated using patch-clamp techniques. In voltage-clamped whole-cells, locally applied GABA (100 microM) evoked a transmembrane chloride current which demonstrated outward rectification. The amplitude of such currents was reversibly suppressed by the GABAA receptor antagonists bicuculline, picrotoxin and RU5135, and enhanced by the general anaesthetic propanidid. Glycine (100 microM) and baclofen (100 microM) were ineffective as agonists. In support of a physiological role for GABA in the adrenal medulla, the co-existence of GABAA and nicotinic acetylcholine (ACh) receptors was demonstrated on whole cells and outside-out membrane patches. Ionophoretically applied GABA reduced the amplitude of depolarization and action potential discharge occurring in response to locally applied ACh (100 microM), but had no effect upon the underlying ACh-induced current. In addition, an excitatory action of GABA was demonstrated by recording action potential waveforms in cell-attached patches. The results are discussed in the context of a GABA-ergic regulation of catecholamine secretion.

MeSH Terms
Acetylcholine/pharmacology Action Potentials/drug effects Adrenal Medulla/physiology Androstanes/pharmacology Animals Azasteroids/pharmacology Bicuculline/pharmacology Cattle Cell Membrane/physiology Chloride Channels Chlorides/physiology Chromaffin System/physiology Electrophysiology GABA Antagonists Membrane Proteins/physiology Picrotoxin/pharmacology Propanidid/pharmacology Receptors, Cholinergic/metabolism Receptors, GABA-A/drug effects,physiology gamma-Aminobutyric Acid/pharmacology
Chemicals
Androstanes Azasteroids Chloride Channels Chlorides GABA Antagonists Membrane Proteins Receptors, Cholinergic Receptors, GABA-A Picrotoxin gamma-Aminobutyric Acid Propanidid Acetylcholine RU 5135 Bicuculline
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Peters J A
Department of Pharmacology and Clinical Pharmacology, Ninewells Hospital, Medical School, University of Dundee, Great Britain.
Lambert J J
Cottrell G A
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Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00373146
Published
1989-10-00
Pages
95-103
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1007/BF00373146 View journal details
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