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PMID: 6472489 Published · ppublish English Journal Article

The nature of the stimulatory action of gamma-aminobutyric acid in the isolated perfused dog adrenals.

Naunyn-Schmiedeberg's archives of pharmacology ·Vol. 326 ·No. 2 ·1984-06-00 ·Pages 106-10

Kitayama S, Morita K, Dohi T, Tsujimoto A

Abstract

The effect of gamma-aminobutyric acid (GABA) on catecholamine (CA) release from adrenal medulla was investigated. GABA and GABA agonists, 3-amino-1-propane-sulfonic acid and imidazole-4-acetic acid caused CA release from isolated perfused dog adrenals in a dose-dependent manner, and no tachyphylaxis to GABA was observed. CA release elicited by GABA was antagonized by bicuculline and picrotoxin. This antagonism was specific for GABA- and GABA agonist-induced responses, response to acetylcholine being unaffected. Pretreatment with atropine plus hexamethonium did not affect the response to GABA. GABA-induced CA release was abolished by the removal of Ca2+ from perfusion medium, but not by the removal of Na+ or Cl-. Verapamil, CoCl2 and dibucaine blocked the effect of GABA. A Na+ channel blocker, tetrodotoxin did not reduce GABA-evoked CA release. These results suggest that GABA may interact with its receptor to evoke CA release from adrenal medulla in a fashion of Ca2+-dependence and independence on external Na+ or Cl-.

MeSH Terms
Acetylcholine/pharmacology Adrenal Glands/drug effects Animals Atropine/pharmacology Bicuculline/pharmacology Calcium/physiology Catecholamines/metabolism Cobalt/pharmacology Dibucaine/pharmacology Dogs Female Hexamethonium Compounds/pharmacology In Vitro Techniques Kinetics Male Picrotoxin/pharmacology Tetrodotoxin/pharmacology Verapamil/pharmacology gamma-Aminobutyric Acid/pharmacology,physiology
Chemicals
Catecholamines Hexamethonium Compounds Picrotoxin Cobalt Tetrodotoxin gamma-Aminobutyric Acid Atropine Verapamil cobaltous chloride Dibucaine Acetylcholine Calcium Bicuculline
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kitayama S
Morita K
Dohi T
Tsujimoto A
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25 references, click to expand
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Article Info
Journal
Naunyn-Schmiedeberg's archives of pharmacology
Abbr.
Naunyn Schmiedebergs Arch Pharmacol
ISSN
0028-1298
DOI
10.1007/BF00517305
Published
1984-06-00
Pages
106-10
Language
English
Region
Germany
NLM ID
0326264
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00517305 View journal details
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