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PMID: 25591940 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

The Arabidopsis alkaline ceramidase TOD1 is a key turgor pressure regulator in plant cells.

Nature communications ·Vol. 6 ·2015-01-16 ·Pages 6030

Chen LY, Shi DQ, Zhang WJ, Tang ZS, Liu J, Yang WC

Abstract

Turgor pressure plays pivotal roles in the growth and movement of walled cells that make up plants and fungi. However, the molecular mechanisms regulating turgor pressure and the coordination between turgor pressure and cell wall remodelling for cell growth remain poorly understood. Here, we report the characterization of Arabidopsis TurgOr regulation Defect 1 (TOD1), which is preferentially expressed in pollen tubes and silique guard cells. We demonstrate that TOD1 is a Golgi-localized alkaline ceramidase. tod1 mutant pollen tubes have higher turgor than wild type and show growth retardation both in pistils and in agarose medium. In addition, tod1 guard cells are insensitive to abscisic acid (ABA)-induced stomatal closure, whereas sphingosine-1-phosphate, a putative downstream component of ABA signalling and product of alkaline ceramidases, promotes closure in both wild type and tod1. Our data suggest that TOD1 acts in turgor pressure regulation in both guard cells and pollen tubes.

MeSH Terms
Alkaline Ceramidase/metabolism Arabidopsis/enzymology,metabolism Arabidopsis Proteins/metabolism Lysophospholipids/metabolism Plant Stomata/metabolism Pollen Tube/enzymology,metabolism Signal Transduction Sphingosine/analogs & derivatives,metabolism
Chemicals
Arabidopsis Proteins Lysophospholipids sphingosine 1-phosphate Alkaline Ceramidase Sphingosine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Chen Li-Yu
1] State Key Laboratory of Molecular Developmental Biology and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China [2] University of Chinese Academy of Sciences, Beijing 100049, China.
Shi Dong-Qiao
State Key Laboratory of Molecular Developmental Biology and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Zhang Wen-Juan
1] State Key Laboratory of Molecular Developmental Biology and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China [2] University of Chinese Academy of Sciences, Beijing 100049, China.
Tang Zuo-Shun
State Key Laboratory of Molecular Developmental Biology and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Liu Jie
State Key Laboratory of Molecular Developmental Biology and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Yang Wei-Cai
1] State Key Laboratory of Molecular Developmental Biology and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China [2] Collaborative Innovation Center for Genetics and Development, Fudan University, Shanghai 200433, China.
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Article Info
Journal
Nature communications
Abbr.
Nat Commun
ISSN
2041-1723
DOI
10.1038/ncomms7030
Published
2015-01-16
Epub
2015-00-16
Pages
6030
Language
English
Region
England
NLM ID
101528555
PMCID
PMC4309442
Subset
IM
Databases
SRA
SRR1652473
External Links
PubMed source DOI: 10.1038/ncomms7030 PMC Full Text View journal details
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