Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues.

Plant molecular biology ·Vol. 5 ·No. 2 ·1985-03-00 ·Pages 69-76

Rogers SO, Bendich AJ

Abstract

We have developed a DNA extraction procedure for milligram amounts of plant tissue. Yields ranged from 0.3-200 nanograms of DNA per milligram of tissue. The factors affecting yield are discussed. Fresh tissue, as well as herbarium specimens (22-118 years old) and mummified seeds and embryos (500 to greater than 44 600 years old) were used. All tissues attempted (57 types from 29 species) yielded measurable amounts of DNA. In no case tested was inhibition observed for restriction enzymes BamHI or EcoRI.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Rogers S O

Department of Botany, University of Washington, 98195, Seattle, WA, U.S.A..

Bendich A J

References (8)

8 references, click to expand

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Article Info

Journal

Plant molecular biology

Abbr.

Plant Mol Biol

ISSN

0167-4412

DOI

10.1007/BF00020088

Published

1985-03-00

Pages

69-76

Language

English

Region

Netherlands

NLM ID

9106343

External Links

PubMed source DOI: 10.1007/BF00020088 View journal details

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Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues.

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