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PMID: 2555158 Published · ppublish English Journal Article

Molecular basis of functional diversity of voltage-gated potassium channels in mammalian brain.

The EMBO journal ·Vol. 8 ·No. 11 ·1989-11-00 ·Pages 3235-44

Stühmer W, Ruppersberg JP, Schröter KH, Sakmann B, Stocker M, Giese KP, Perschke A, Baumann A, Pongs O

Abstract

Cloning and sequencing of cDNAs isolated from a rat cortex cDNA library reveals that a gene family encodes several highly homologous K+ channel forming (RCK) proteins. Functional characterization of the channels expressed in Xenopus laevis oocytes following microinjection of in vitro transcribed RCK-specific RNAs shows that each of the RCK proteins forms K+ channels that differ greatly in both their functional and pharmacological properties. This suggests that the molecular basis for the diversity of voltage-gated K+ channels in mammalian brain is based, at least partly, on the expression of several RCK proteins by a family of genes and their assembly to homooligomeric K+ channels with different functional properties.

MeSH Terms
4-Aminopyridine/pharmacology Amino Acid Sequence Animals Base Sequence Blotting, Southern Brain/metabolism Cloning, Molecular DNA/genetics Electric Conductivity Electrophysiology Gene Expression Regulation Ion Channel Gating Molecular Sequence Data Multigene Family Nerve Tissue Proteins/genetics,metabolism Potassium Channels/metabolism Rats Sequence Homology, Nucleic Acid Tetraethylammonium Compounds/pharmacology Xenopus laevis
Chemicals
Nerve Tissue Proteins Potassium Channels Tetraethylammonium Compounds DNA 4-Aminopyridine
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Stühmer W
Max-Planck-Institut für biophysikalische Chemie, Abteilung Membranbiophysik, Göttingen, FRG.
Ruppersberg J P
Schröter K H
Sakmann B
Stocker M
Giese K P
Perschke A
Baumann A
Pongs O
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-11-00
Pages
3235-44
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401447
Subset
IM
Grants
Medical Research Council · G0800393 · United Kingdom
Databases
GENBANK
X16001, X16002, X16003
External Links
PubMed source PMC Full Text View journal details
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