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PMID: 2456579 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A-type potassium channels expressed from Shaker locus cDNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 15 ·1988-08-00 ·Pages 5723-7

Iverson LE, Tanouye MA, Lester HA, Davidson N, Rudy B

Abstract

A-type K+ currents are expressed in Xenopus oocytes injected with in vitro-synthesized transcripts from cDNAs for the Drosophila Shaker (Sh) locus. A single Sh gene product, possibly as a multimer, is sufficient for formation of functional A channels. Various Sh RNAs express A currents with distinct kinetic properties. An analysis of structure-function relationships shows that the conserved central region of Sh polypeptides determines ionic selectivity and overall channel behavior, whereas the divergent amino and carboxyl termini can modify channel kinetics. Alternative splicing of Sh gene transcripts may provide one mechanism for the generation of K+ channel diversity.

MeSH Terms
Animals DNA/genetics Drosophila Electrophysiology Genes Ion Channels/metabolism Kinetics Membrane Potentials Oocytes Potassium/metabolism RNA/genetics Transcription, Genetic Xenopus laevis
Chemicals
Ion Channels RNA DNA Potassium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Iverson L E
Division of Biology, California Institute of Technology, Pasadena 91125.
Tanouye M A
Lester H A
Davidson N
Rudy B
References (15)
15 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.15.5723
Published
1988-08-00
Pages
5723-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC281833
Subset
IM
Grants
NIGMS NIH HHS · GM10991 · United States
NIGMS NIH HHS · GM26976 · United States
NINDS NIH HHS · NS21327 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.15.5723 PMC Full Text View journal details
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