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PMID: 2550758 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence and regulation of expression of the Aspergillus nidulans gdhA gene encoding NADP dependent glutamate dehydrogenase.

Molecular & general genetics : MGG ·Vol. 218 ·No. 1 ·1989-07-00 ·Pages 105-11

Hawkins AR, Gurr SJ, Montague P, Kinghorn JR

Abstract

The nucleotide sequence of the Aspergillus nidulans gdhA gene encoding NADP linked glutamate dehydrogenase has been determined and Northern blot analysis used to study the regulation of expression of this gene. The gdhA gene is 1485 nucleotides long and, by comparison with the corresponding Neurospora crassa am gene, has two putative introns of 53 nucleotides and a protein encoding region of 1380 nucleotides that codes for an inferred protein of 49.63 kDa which shows regions of homology with glutamate dehydrogenase proteins from a range of organisms. mRNA analysis of wild-type mycelium grown under a variety of conditions shows that: (a) the highest levels are seen with glucose as the carbon source with inorganic nitrogen; and (b) no gdhA mRNA is detectable when cells are transferred to amino acids as sole carbon source, closely matching the observed glutamate dehydrogenase activity levels under identical conditions. The results presented strongly suggest that a good carbon source is a prerequisite for transcription, but the molecular mechanism responsible is unclear.

MeSH Terms
Amino Acid Sequence Aspergillus nidulans/enzymology,genetics Base Sequence Blotting, Northern Endonucleases/genetics Gene Expression Regulation Genes, Fungal Glutamate Dehydrogenase/biosynthesis,genetics Glutamate Dehydrogenase (NADP+) Introns Molecular Sequence Data Promoter Regions, Genetic RNA, Messenger/genetics Sequence Homology, Nucleic Acid Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic
Chemicals
RNA, Messenger Glutamate Dehydrogenase Glutamate Dehydrogenase (NADP+) Endonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hawkins A R
Department of Biochemistry and Genetics, University of Newcastle upon Tyne, UK.
Gurr S J
Montague P
Kinghorn J R
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330572
Published
1989-07-00
Pages
105-11
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1007/BF00330572
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