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PMID: 2987645 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The Klebsiella aerogenes glutamate dehydrogenase (gdhA) gene: cloning, high-level expression and hybrid enzyme formation in Escherichia coli.

Molecular & general genetics : MGG ·Vol. 199 ·No. 1 ·1985-00-00 ·Pages 141-5

Mountain A, McPherson MJ, Baron AJ, Wootton JC

Abstract

The NADP-dependent glutamate dehydrogenase gene of Klebsiella aerogenes was cloned in E. coli in the expression plasmid pRK9. The cloned gene shows a high level of expression in E. coli in the hybrid plasmid pKG3 and such expression is independent of the vector promoter, as shown by experiments in which the promoter was deleted. Active hybrid GDH hexamers were shown in cell-free extracts of an E. coli strain carrying cloned gdhA genes of both E. coli and K. aerogenes. The nucleotide sequence of the N-terminal coding region of the K. aerogenes gdhA gene was determined and found to be strongly homologous with that of E. coli.

MeSH Terms
Base Sequence Chromosome Mapping DNA Restriction Enzymes Escherichia coli/genetics Gene Expression Regulation Genes Genes, Bacterial Glutamate Dehydrogenase/genetics Klebsiella pneumoniae/enzymology,genetics Macromolecular Substances Neurospora crassa/genetics
Chemicals
Macromolecular Substances Glutamate Dehydrogenase DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Mountain A
McPherson M J
Baron A J
Wootton J C
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30 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00327523
Published
1985-00-00
Pages
141-5
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00327523
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