Re-assessment of ammonium-ion affinities of NADP-specific glutamate dehydrogenases. Activation of the Neurospora crassa enzyme by ammonium and rubidium ions.
The NADP-specific glutamate dehydrogenase of Neurospora crassa shows complex interactions with NH4+ ions, characterized by biphasic downwardly convex double-reciprocal plots. These kinetics are explained by the action of NH4+ both as a substrate and, acting at a separate cation-binding site, as an activator. Rb+ ions, and to a smaller extent other univalent cations, also activate by acting as analogues of NH4+. Previous failure to recognize this effect, which probably also occurs in homologous enzymes from some other species, has led to significant overestimates in published reports of the Km for NH4+ of some NADP-specific glutamate dehydrogenases.
16 references, click to expand
-
A further genetic variety of glutamic acid dehydrogenase in Neurospora crassa.Biochem J. 1960 Oct;77:96-105 PMID: 13699528
-
Glutamate dehydrogenases from Chlorella: forms, regulation and properties.Mol Cell Biochem. 1977 May 3;15(3):201-12 PMID: 18663
-
Localisation of a strongly conserved section of coding sequence in glutamate dehydrogenase genes.FEBS Lett. 1982 Oct 4;147(1):21-5 PMID: 6754449
-
Affinity chromatography of the Neurospora NADP-specific glutamate dehydrogenase, its mutational variants and hybrid hexamers.Biochem J. 1977 Oct 1;167(1):95-108 PMID: 22328
-
Re-investigation of the effects of L-glutamine and L-asparagine on the Neurospora crassa NADP-specific glutamate dehydrogenase.Biochem J. 1976 Dec 1;159(3):803-6 PMID: 137720
-
Studies on the kinetics and regulation of glutamate dehydrogenase of Salmonella typhimurium.Can J Microbiol. 1973 Apr;19(4):439-50 PMID: 4144744
-
Regulation of the assimilation of nitrogen compounds.Annu Rev Biochem. 1978;47:1127-62 PMID: 28074
-
Kinetic mechanism of Escherichia coli glutamine synthetase.Biochemistry. 1980 Nov 25;19(24):5513-9 PMID: 6109545
-
Stimulus-permeability coupling: role of calcium in the receptor regulation of membrane permeability.Pharmacol Rev. 1978 Jun;30(2):209-45 PMID: 224401
-
The amino acid sequence of Neurospora NADP-specific glutamate dehydrogenase. Peptic and chymotryptic peptides and the complete sequence.Biochem J. 1975 Sep;149(3):757-73 PMID: 1002
-
The occurrence of two different glutamic dehydrogenases in Neurospora.Can J Microbiol. 1961 Jun;7:319-28 PMID: 13746409
-
Mutational amino acid replacements in Neurospora crassa NADP-specific glutamate dehydrogenase.J Mol Biol. 1976 Sep 5;106(1):1-22 PMID: 9517
-
Slow conformational changes of a Neurospora glutamate dehydrogenase studied by protein fluorescence.Biochem J. 1974 Nov;143(2):317-29 PMID: 4156826
-
The effect of pH on the affinities of enzymes for substrates and inhibitors.Biochem J. 1953 Aug;55(1):161-70 PMID: 13093634
-
Mutational variants of the Neurospora crassa NADP-specific glutamate dehydrogenase altered in a conformational equilibrium.J Mol Biol. 1981 Jul 5;149(3):521-40 PMID: 6273586
-
Subunit ratios of separated hybrid hexamers of Neurospora NADP-specific glutamate dehydrogenase containing complementing mutationally modified monomers.Biochem J. 1978 Dec 1;175(3):1125-33 PMID: 33665
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
