Re-investigation of the effects of L-glutamine and L-asparagine on the Neurospora crassa NADP-specific glutamate dehydrogenase.

The Biochemical journal ·Vol. 159 ·No. 3 ·1976-12-01 ·Pages 803-6

Wootton JC, Kavanagh JP, Baron AJ, Lovett MG

Abstract

L-Glutamine, when purified free of traces of NH4+ present in solution, does not act as an alternative substrate to NH4+ for the NADP-specific glutamate dehydrogenase of Neurospora. L-Glutamine interferes with detection of small quantities of NH4+ by Nessler's reagent. L-Asparagine is not an alternative substrate to NH4+ for this enzyme.

MeSH Terms

Asparagine/metabolism Chromatography, Ion Exchange Glutamate Dehydrogenase/metabolism Glutamine/metabolism Neurospora/enzymology Neurospora crassa/enzymology Quaternary Ammonium Compounds/metabolism

Chemicals

Quaternary Ammonium Compounds Glutamine Asparagine Glutamate Dehydrogenase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Wootton J C

Kavanagh J P

Baron A J

Lovett M G

References (6)

6 references, click to expand

A further genetic variety of glutamic acid dehydrogenase in Neurospora crassa.
Biochem J. 1960 Oct;77:96-105 PMID: 13699528
The occurrence of two different glutamic dehydrogenases in Neurospora.
Can J Microbiol. 1961 Jun;7:319-28 PMID: 13746409
On the utilization of L-glutamine by glutamate dehydrogenase.
Biochem Biophys Res Commun. 1976 May 17;70(2):373-80 PMID: 180990
Alternative substrates for glutamate dehydrogenases.
Biochem Biophys Res Commun. 1975 Jan 6;62(1):78-84 PMID: 234220
Slow conformational changes of a Neurospora glutamate dehydrogenase studied by protein fluorescence.
Biochem J. 1974 Nov;143(2):317-29 PMID: 4156826
Regulation of glutamate dehydrogenases during morphogenesis of Schizophyllum commune.
J Bacteriol. 1967 Mar;93(3):904-13 PMID: 4381636

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1590803

Published

1976-12-01

Pages

803-6

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1164185

Subset

External Links

PubMed source DOI: 10.1042/bj1590803 PMC Full Text View journal details

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Re-investigation of the effects of L-glutamine and L-asparagine on the Neurospora crassa NADP-specific glutamate dehydrogenase.

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