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PMID: 2538801 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Epstein-Barr virus episome-based promoter function in human myeloid cells.

Nucleic acids research ·Vol. 17 ·No. 5 ·1989-03-11 ·Pages 1989-2003

Hauer CA, Getty RR, Tykocinski ML

Abstract

Epstein-Barr virus (EBV) episomal replicons offer an expeditious means for amplifying transfected genes in human cells. A panel of EBV episomes was constructed to assess the relative utility of five distinct eukaryotic promoter elements for high level and inducible gene expression in stably transfected human myeloid leukemia cells. The Rous sarcoma virus 3' long terminal repeat (LTR) was most highly suited for EBV episome-based gene expression, whereas the lymphopapilloma virus and the SV40 early regulatory elements exhibited substantially lower activities. Chemically responsive promoter elements, such as the SV40 early, human metallothionein IIA and rat GRP78 gene promoters, retained their inducibility when EBV episome-based. Differences in gene expression obtained with the episomes reflected differential promoter activity rather than significant variations in episome copy numbers per cell. These observations provide guidelines for the optimal design of EBV episomal expression vectors for human expression work.

MeSH Terms
Cell Differentiation/drug effects Cell Line Cell-Free System Chloramphenicol O-Acetyltransferase Endoplasmic Reticulum Chaperone BiP Genes, Viral Herpesvirus 4, Human/genetics Humans Leukemia, Myeloid/genetics Promoter Regions, Genetic Replicon Tetradecanoylphorbol Acetate Transfection
Chemicals
Endoplasmic Reticulum Chaperone BiP HSPA5 protein, human Chloramphenicol O-Acetyltransferase Tetradecanoylphorbol Acetate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hauer C A
Institute of Pathology, Case Western Reserve University, Cleveland, OH 44106.
Getty R R
Tykocinski M L
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.5.1989
Published
1989-03-11
Pages
1989-2003
Language
English
Region
England
NLM ID
0411011
PMCID
PMC317538
Subset
IM
Grants
NCI NIH HHS · P30 CA 43703 · United States
NCI NIH HHS · R29 CA 47566 · United States
External Links
PubMed source DOI: 10.1093/nar/17.5.1989 PMC Full Text View journal details
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