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PMID: 3357885 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Phorbol ester-induced terminal differentiation is inhibited in human U-937 monoblastic cells expressing a v-myc oncogene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 8 ·1988-04-00 ·Pages 2638-42

Larsson LG, Ivhed I, Gidlund M, Pettersson U, Vennström B, Nilsson K

Abstract

Induction of differentiation of the human monoblastic cell line U-937 in vitro by several physiologic and nonphysiologic inducers is accompanied by a rapid decrease in expression of MYC, the endogenous human myc protooncogene. To investigate whether this reduction is a prerequisite for terminal differentiation, we introduced a constitutively expressed v-myc gene into U-937 cells. The results show that constitutive expression of an avian v-myc oncogene does not interfere with phorbol 12-myristate 13-acetate-induced differentiation of U-937 cells early after stimulation. However, after 24 hr the differentiation process is reversed, as judged by a full recovery of the proliferative capacity and reexpression of the immature phenotype, within the next 2-4 days. We conclude that the terminal stage of macrophage differentiation is inhibited in U-937 cells constitutively expressing a v-myc oncogene.

MeSH Terms
Antibody-Dependent Cell Cytotoxicity Cell Adhesion Cell Differentiation/drug effects Cell Division/drug effects Cell Line Gene Expression Regulation Humans Killer Cells, Natural/immunology Monocytes/cytology,immunology Oncogene Proteins, Viral/genetics Oncogenes RNA, Messenger/genetics Tetradecanoylphorbol Acetate/pharmacology
Chemicals
Oncogene Proteins, Viral RNA, Messenger Tetradecanoylphorbol Acetate
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Larsson L G
Department of Pathology, University of Uppsala, University Hospital, Sweden.
Ivhed I
Gidlund M
Pettersson U
Vennström B
Nilsson K
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.8.2638
Published
1988-04-00
Pages
2638-42
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC280053
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.85.8.2638 PMC Full Text View journal details
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