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PMID: 6526015 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Transformation of mammalian fibroblasts and macrophages in vitro by a murine retrovirus encoding an avian v-myc oncogene.

The EMBO journal ·Vol. 3 ·No. 13 ·1984-12-20 ·Pages 3223-9

Vennström B, Kahn P, Adkins B, Enrietto P, Hayman MJ, Graf T, Luciw P

Abstract

A murine retrovirus which expresses the avain v-myc OK10 oncogene was constructed. The virus, denoted MMCV, readily transforms fibroblasts of established lines, such as mouse NIH/3T3 and rat 208F cells, to anchorage-independent growth in agarose. The virus also transforms primary mouse cells: (i) virus-infected macrophages are induced to form large colonies in semi-solid media, and can easily be expanded into mass cultures; (ii) MMCV-infected fibroblastic cells from mouse limb buds undergo morphological transformation and grow in semi-solid medium. MMCV thus transforms both mouse fibroblastic cells and macrophages in vitro, in a fashion similar to the v-myc-containing avian viruses in chicken cells. The possibility of introducing a transforming myc gene into mammalian cells by virus infection provides a novel approach for studying the mechanism of myc transformation in cells from many lineages.

MeSH Terms
Animals Cell Line Cell Transformation, Neoplastic Cell Transformation, Viral Fibroblasts Genes, Viral Macrophages Mice Oncogenes Rats Retroviridae/genetics
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Vennström B
Kahn P
Adkins B
Enrietto P
Hayman M J
Graf T
Luciw P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-12-20
Pages
3223-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557841
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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