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PMID: 2536405 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Endothelin stimulates phospholipase C, Na+/H+ exchange, c-fos expression, and mitogenesis in rat mesangial cells.

The Journal of clinical investigation ·Vol. 83 ·No. 2 ·1989-02-00 ·Pages 708-12

Simonson MS, Wann S, Mené P, Dubyak GR, Kester M, Nakazato Y, Sedor JR, Dunn MJ

Abstract

A recently described peptide hormone, endothelin, is a potent vasoconstrictor, but it is unclear whether endothelin has other biological actions. These experiments extend the range of biological actions of endothelin to stimulation of mitogenesis. Endothelin at low concentrations (0.1-10 nM) induced mitogenesis by quiescent rat glomerular mesangial cells in culture. Mitogenesis induced by endothelin was accompanied by activation of phospholipase C with increased inositol phosphate turnover and increments of intracellular [Ca2+]. Endothelin also activated Na+/H+ exchange, causing cytosolic alkalinization, and enhanced transcription of the c-fos protooncogene, additional biochemical signals closely linked to proliferation. In addition to being a vasoconstrictor, endothelin thus also functions as a mitogen, presumably through activation of phospholipase C.

MeSH Terms
Animals Carrier Proteins/metabolism Endothelins Gene Expression Regulation/drug effects Glomerular Mesangium/drug effects,metabolism Inositol Phosphates/metabolism Mitosis/drug effects Peptides/pharmacology Proto-Oncogenes Rats Sodium-Hydrogen Exchangers Type C Phospholipases/metabolism
Chemicals
Carrier Proteins Endothelins Inositol Phosphates Peptides Sodium-Hydrogen Exchangers Type C Phospholipases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Simonson M S
Department of Medicine, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.
Wann S
Mené P
Dubyak G R
Kester M
Nakazato Y
Sedor J R
Dunn M J
References (34)
34 references, click to expand
  1. Rapid formation of inositol 1,3,4,5-tetrakisphosphate and inositol 1,3,4-trisphosphate in rat parotid glands may both result indirectly from receptor-stimulated release of inositol 1,4,5-trisphosphate from phosphatidylinositol 4,5-bisphosphate.
    Biochem J. 1986 Sep 1;238(2):507-16 PMID: 3026354
  2. Inositol lipids and cell proliferation.
    Biochim Biophys Acta. 1987 Apr 20;907(1):33-45 PMID: 3032258
  3. The interleukin 1 gene is expressed by rat glomerular mesangial cells and is augmented in immune complex glomerulonephritis.
    J Immunol. 1987 May 15;138(10):3207-12 PMID: 2883234
  4. Primary structure of the receptor for calcium channel blockers from skeletal muscle.
    Nature. 1987 Jul 23-29;328(6128):313-8 PMID: 3037387
  5. Inositol trisphosphate and diacylglycerol: two interacting second messengers.
    Annu Rev Biochem. 1987;56:159-93 PMID: 3304132
  6. Elevation of cytosolic free calcium by platelet-activating factor in cultured rat mesangial cells.
    FASEB J. 1987 Sep;1(3):215-9 PMID: 2442057
  7. Effects of PDGF on inositol phosphates, Ca2+, and contraction of mesangial cells.
    Am J Physiol. 1987 Sep;253(3 Pt 2):F458-63 PMID: 3631281
  8. The glomerular mesangial cell: an expanding role for a specialized pericyte.
    FASEB J. 1987 Oct;1(4):272-81 PMID: 3308611
  9. Direct measurements of cell number using computer-aided video microscopy.
    Anal Biochem. 1987 Nov 15;167(1):106-12 PMID: 3434786
  10. A novel potent vasoconstrictor peptide produced by vascular endothelial cells.
    Nature. 1988 Mar 31;332(6163):411-5 PMID: 2451132
  11. Cloning and sequence analysis of cDNA encoding the precursor of a human endothelium-derived vasoconstrictor peptide, endothelin: identity of human and porcine endothelin.
    FEBS Lett. 1988 Apr 25;231(2):440-4 PMID: 3282927
  12. Highly cooperative opening of calcium channels by inositol 1,4,5-trisphosphate.
    Science. 1988 Apr 29;240(4852):653-6 PMID: 2452482
  13. Bradykinin stimulates a rise in cytosolic calcium in renal glomerular mesangial cells via a pertussis toxin insensitive pathway.
    Can J Physiol Pharmacol. 1988 Jan;66(1):43-8 PMID: 3370534
  14. Mitogens and ion fluxes.
    Annu Rev Physiol. 1988;50:207-23 PMID: 2454071
  15. Cellular mechanism of action by a novel vasoconstrictor endothelin in cultured rat vascular smooth muscle cells.
    Biochem Biophys Res Commun. 1988 Aug 15;154(3):868-75 PMID: 3044368
  16. Identification and transmembrane signaling of leukotriene D4 receptors in human mesangial cells.
    Am J Physiol. 1988 Dec;255(6 Pt 1):C771-80 PMID: 2849305
  17. Microelisa reader quantitation of fixed, stained, solubilized cells in microtitre dishes.
    Biotechniques. 1988 May;6(5):418-9 PMID: 2483658
  18. Early signals in the mitogenic response.
    Science. 1986 Oct 10;234(4773):161-6 PMID: 3018928
  19. Involvement of three intracellular messenger systems, protein kinase C, calcium ion and cyclic AMP, in the regulation of c-fos gene expression in Swiss 3T3 cells.
    FEBS Lett. 1986 Nov 10;208(1):39-42 PMID: 3021538
  20. The metabolism of phosphoinositide-derived messenger molecules.
    Science. 1986 Dec 19;234(4783):1519-26 PMID: 3024320
  21. Measurements of cation transport with metallochromic indicators.
    Methods Enzymol. 1979;56:301-38 PMID: 37412
  22. Two types of scorpion neurotoxins characterized by their binding to two separate receptor sites on rat brain synaptosomes.
    Biochem Biophys Res Commun. 1980 Aug 29;95(4):1607-14 PMID: 7417336
  23. Cytoplasmic pH and free Mg2+ in lymphocytes.
    J Cell Biol. 1982 Oct;95(1):189-96 PMID: 6815204
  24. Na+/H+ antiport in Swiss 3T3 cells: mitogenic stimulation leads to cytoplasmic alkalinization.
    Proc Natl Acad Sci U S A. 1982 Dec;79(24):7778-82 PMID: 6961450
  25. Role of endothelium in responses of vascular smooth muscle.
    Circ Res. 1983 Nov;53(5):557-73 PMID: 6313250
  26. Expression of alpha subunit and luteinizing hormone beta genes in the ovine anterior pituitary. Estradiol suppresses accumulation of mRNAS for both alpha subunit and luteinizing hormone beta.
    J Biol Chem. 1983 Oct 25;258(20):12087-90 PMID: 6195150
  27. A new generation of Ca2+ indicators with greatly improved fluorescence properties.
    J Biol Chem. 1985 Mar 25;260(6):3440-50 PMID: 3838314
  28. Glomerular cell culture.
    Lab Invest. 1985 Aug;53(2):122-31 PMID: 3894792
  29. Intracellular Ca2+ mobilization activated by extracellular ATP in Ehrlich ascites tumor cells.
    J Biol Chem. 1985 Sep 5;260(19):10653-61 PMID: 4030763
  30. The pathogenesis of atherosclerosis--an update.
    N Engl J Med. 1986 Feb 20;314(8):488-500 PMID: 3511384
  31. Modulation of vascular smooth muscle contraction by the endothelium.
    Annu Rev Physiol. 1986;48:307-20 PMID: 2871807
  32. Effects of growth factors on intracellular pH regulation.
    Annu Rev Physiol. 1986;48:363-76 PMID: 3010817
  33. Inducible production of c-fos antisense RNA inhibits 3T3 cell proliferation.
    Proc Natl Acad Sci U S A. 1986 Jul;83(13):4794-8 PMID: 3523478
  34. Vasopressin increases cytosolic free calcium concentration in glomerular mesangial cells.
    Am J Physiol. 1986 Jul;251(1 Pt 2):F94-102 PMID: 3014901
Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI113935
Published
1989-02-00
Pages
708-12
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC303732
Subset
IM
Grants
NIGMS NIH HHS · GM-36387 · United States
NHLBI NIH HHS · HL-22563 · United States
NHLBI NIH HHS · HL-37117 · United States
External Links
PubMed source DOI: 10.1172/JCI113935 PMC Full Text View journal details
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