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PMID: 2535730 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Asp-286----Asn-286 in polyomavirus large T antigen relaxes the specificity of binding to the polyomavirus origin.

Journal of virology ·Vol. 63 ·No. 1 ·1989-01-00 ·Pages 242-9

Tang WJ, Folk WR

Abstract

We isolated revertants of a polyomavirus whose origin of DNA replication contains a point mutation in the palindrome to which large T antigen binds. Four independent second-site revertants contain an Asp-286----Asn-286 substitution in large T antigen. This mutant large T antigen activates replication of DNAs containing the mutant polyomavirus origin as well as replication of DNAs containing the wild-type origin; however, replication of DNAs with enhancer mutations is not activated by this large T antigen. The Asn-286 mutation occurs in a positively charge region of large T antigen near the location of several mutations which inactivate DNA replication. We suggest that this region of large T antigen is responsible for recognition of specific DNA sequences at the origin and that ionic forces are important for this interaction.

MeSH Terms
Amino Acid Sequence Antigens, Polyomavirus Transforming/biosynthesis,genetics Base Sequence DNA Replication DNA, Viral/genetics Gene Expression Regulation Molecular Sequence Data Mutation Phenotype Polyomavirus/genetics,immunology,metabolism Virus Replication
Chemicals
Antigens, Polyomavirus Transforming DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tang W J
Department of Microbiology, University of Texas, Austin 78712-1095.
Folk W R
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.1.242-249.1989
Published
1989-01-00
Pages
242-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC247678
Subset
IM
Grants
NCI NIH HHS · CA 38538 · United States
NCI NIH HHS · CA 45033 · United States
External Links
PubMed source DOI: 10.1128/JVI.63.1.242-249.1989 PMC Full Text View journal details
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