Cloning of a gene encoding a major secreted polypeptide of Listeria monocytogenes and its potential use as a species-specific probe.

Applied and environmental microbiology ·Vol. 55 ·No. 9 ·1989-09-00 ·Pages 2251-6

Flamm RK, Hinrichs DJ, Thomashow MF

Abstract

A gene, designated msp, that encodes a major secreted polypeptide with a molecular mass of approximately 60 kilodaltons (kDa) was cloned from Listeria monocytogenes 10403. DNA hybridization analysis indicated that the msp gene was highly conserved among 15 independent L. monocytogenes isolates and that each of 5 isolates tested secreted a 60-kDa polypeptide that was immunologically related to the msp gene product. DNA sequences related to msp were not detected in any other Listeria species or in strains of Bacillus cereus, Bacillus thuringiensis, Streptococcus pyogenes, or Streptococcus pneumoniae when standard stringent DNA hybridization conditions were used. Under nonstringent conditions, related sequences were detected in Listeria ivanovii, Listeria seeligeri, and Listeria innocua, and immunoblot analysis indicated that these strains secreted polypeptides of about 60 kDa that were immunologically related to the msp gene product. The possibility of using the msp gene as a probe for the detection of L. monocytogenes and the potential functions of the msp gene product are discussed.

MeSH Terms

Bacterial Proteins/biosynthesis,genetics Base Sequence Cloning, Molecular DNA Probes DNA, Bacterial/isolation & purification Genes, Bacterial Listeria monocytogenes/genetics,metabolism Nucleic Acid Hybridization Peptide Biosynthesis Peptides/genetics Plasmids Sequence Homology, Nucleic Acid Species Specificity Transcription, Genetic

Chemicals

Bacterial Proteins DNA Probes DNA, Bacterial Peptides

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Flamm R K

Department of Microbiology, Washington State University, Pullman 99164.

Hinrichs D J

Thomashow M F

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22 references, click to expand

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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.55.9.2251-2256.1989

Published

1989-09-00

Pages

2251-6

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC203064

Subset

External Links

PubMed source DOI: 10.1128/aem.55.9.2251-2256.1989 PMC Full Text View journal details

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Cloning of a gene encoding a major secreted polypeptide of Listeria monocytogenes and its potential use as a species-specific probe.

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