Detection of hemolytic Listeria monocytogenes by using DNA colony hybridization.

Applied and environmental microbiology ·Vol. 53 ·No. 9 ·1987-09-00 ·Pages 2256-9

Datta AR, Wentz BA, Hill WE

Abstract

A fragment of about 500 base pairs of the beta-hemolysin gene from Listeria monocytogenes was used to screen different bacterial strains by DNA colony hybridization. The cells in the colonies were lysed by microwaves in the presence of sodium hydroxide. Of 52 different strains of Listeria species screened, only the DNA from beta-hemolytic (CAMP-positive) strains of L. monocytogenes hybridized with this probe.

MeSH Terms

Autoradiography Centrifugation, Density Gradient DNA Restriction Enzymes DNA, Bacterial/analysis Deoxyribonuclease HindIII Deoxyribonucleases, Type II Site-Specific Genes, Bacterial Hemolysin Proteins/genetics Listeria monocytogenes/genetics,isolation & purification,radiation effects Microwaves Nucleic Acid Hybridization

Chemicals

DNA, Bacterial Hemolysin Proteins DNA Restriction Enzymes Deoxyribonuclease HindIII Deoxyribonucleases, Type II Site-Specific GTYRAC-specific type II deoxyribonucleases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Datta A R

Division of Microbiology, Food and Drug Administration, Washington, D.C. 20204.

Wentz B A

Hill W E

References (13)

13 references, click to expand

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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.53.9.2256-2259.1987

Published

1987-09-00

Pages

2256-9

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC204091

Subset

External Links

PubMed source DOI: 10.1128/aem.53.9.2256-2259.1987 PMC Full Text View journal details

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Detection of hemolytic Listeria monocytogenes by using DNA colony hybridization.

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