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PMID: 24863435 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tomato PYR/PYL/RCAR abscisic acid receptors show high expression in root, differential sensitivity to the abscisic acid agonist quinabactin, and the capability to enhance plant drought resistance.

Journal of experimental botany ·Vol. 65 ·No. 15 ·2014-08-00 ·Pages 4451-64

González-Guzmán M, Rodríguez L, Lorenzo-Orts L, Pons C, Sarrión-Perdigones A, Fernández MA, Peirats-Llobet M, Forment J, Moreno-Alvero M, Cutler SR, Albert A, Granell A, Rodríguez PL

Abstract

Abscisic acid (ABA) plays a crucial role in the plant's response to both biotic and abiotic stress. Sustainable production of food faces several key challenges, particularly the generation of new varieties with improved water use efficiency and drought tolerance. Different studies have shown the potential applications of Arabidopsis PYR/PYL/RCAR ABA receptors to enhance plant drought resistance. Consequently the functional characterization of orthologous genes in crops holds promise for agriculture. The full set of tomato (Solanum lycopersicum) PYR/PYL/RCAR ABA receptors have been identified here. From the 15 putative tomato ABA receptors, 14 of them could be grouped in three subfamilies that correlated well with corresponding Arabidopsis subfamilies. High levels of expression of PYR/PYL/RCAR genes was found in tomato root, and some genes showed predominant expression in leaf and fruit tissues. Functional characterization of tomato receptors was performed through interaction assays with Arabidopsis and tomato clade A protein phosphatase type 2Cs (PP2Cs) as well as phosphatase inhibition studies. Tomato receptors were able to inhibit the activity of clade A PP2Cs differentially in an ABA-dependent manner, and at least three receptors were sensitive to the ABA agonist quinabactin, which inhibited tomato seed germination. Indeed, the chemical activation of ABA signalling induced by quinabactin was able to activate stress-responsive genes. Both dimeric and monomeric tomato receptors were functional in Arabidopsis plant cells, but only overexpression of monomeric-type receptors conferred enhanced drought resistance. In summary, gene expression analyses, and chemical and transgenic approaches revealed distinct properties of tomato PYR/PYL/RCAR ABA receptors that might have biotechnological implications.

Keywords
Abscisic acid (ABA) drought resistance tomato ABA receptor tomato clade A PP2C.
MeSH Terms
Abscisic Acid/agonists,metabolism Adaptation, Physiological Arabidopsis Arabidopsis Proteins/metabolism Carrier Proteins/metabolism Droughts Gene Expression Regulation, Plant Genome, Plant Intracellular Signaling Peptides and Proteins Lycopersicon esculentum/metabolism Membrane Transport Proteins/metabolism Phosphoprotein Phosphatases/antagonists & inhibitors Plant Proteins/metabolism Plant Roots/metabolism Protein Phosphatase 2C Quinolones/metabolism Sulfonamides/metabolism
Chemicals
Arabidopsis Proteins Carrier Proteins Intracellular Signaling Peptides and Proteins Membrane Transport Proteins Plant Proteins Pyr1 protein, Arabidopsis Quinolones RCAR1 protein, Arabidopsis Sulfonamides quinabactin Abscisic Acid Phosphoprotein Phosphatases Protein Phosphatase 2C
Authors & Affiliations
13 authors, click to expand affiliations / ORCID
González-Guzmán Miguel
Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Cientificas-Universidad Politecnica de Valencia, ES-46022 Valencia, Spain.
Rodríguez Lesia
Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Cientificas-Universidad Politecnica de Valencia, ES-46022 Valencia, Spain.
Lorenzo-Orts Laura
Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Cientificas-Universidad Politecnica de Valencia, ES-46022 Valencia, Spain.
Pons Clara
Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Cientificas-Universidad Politecnica de Valencia, ES-46022 Valencia, Spain.
Sarrión-Perdigones Alejandro
Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Cientificas-Universidad Politecnica de Valencia, ES-46022 Valencia, Spain.
Fernández Maria A
Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Cientificas-Universidad Politecnica de Valencia, ES-46022 Valencia, Spain.
Peirats-Llobet Marta
Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Cientificas-Universidad Politecnica de Valencia, ES-46022 Valencia, Spain.
Forment Javier
Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Cientificas-Universidad Politecnica de Valencia, ES-46022 Valencia, Spain.
Moreno-Alvero Maria
Departamento de Cristalografía y Biología Estructural, Instituto de Química Física 'Rocasolano', CSIC, Serrano 119, E-28006 Madrid, Spain.
Cutler Sean R
Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, CA 92521, USA.
Albert Armando
Departamento de Cristalografía y Biología Estructural, Instituto de Química Física 'Rocasolano', CSIC, Serrano 119, E-28006 Madrid, Spain.
Granell Antonio
Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Cientificas-Universidad Politecnica de Valencia, ES-46022 Valencia, Spain.
Rodríguez Pedro L
Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Cientificas-Universidad Politecnica de Valencia, ES-46022 Valencia, Spain prodriguez@ibmcp.upv.es.
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Article Info
Journal
Journal of experimental botany
Abbr.
J Exp Bot
ISSN
1460-2431
DOI
10.1093/jxb/eru219
Email
prodriguez@ibmcp.upv.es
Published
2014-08-00
Epub
2014-00-26
Pages
4451-64
Language
English
Region
England
NLM ID
9882906
PMCID
PMC4112642
Subset
IM
External Links
PubMed source DOI: 10.1093/jxb/eru219 PMC Full Text View journal details
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