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PMID: 23934343 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interactions between soybean ABA receptors and type 2C protein phosphatases.

Plant molecular biology ·Vol. 83 ·No. 6 ·2013-12-00 ·Pages 651-64

Bai G, Yang DH, Zhao Y, Ha S, Yang F, Ma J, Gao XS, Wang ZM, Zhu JK

Abstract

The plant hormone abscisic acid (ABA) plays important roles in regulating plant growth, development, and responses to environmental stresses. Proteins in the PYR/PYL/RCAR family (hereafter referred to as PYLs) are known as ABA receptors. Since most studies thus far have focused on Arabidopsis PYLs, little is known about PYL homologs in crop plants. We report here the characterization of 21 PYL homologs (GmPYLs) in soybean. Twenty-three putative GmPYLs can be found from soybean genome sequence and categorized into three subgroups. GmPYLs interact with AtABI1 and two GmPP2Cs in diverse manners. A lot of the subgroup I GmPYLs interact with PP2Cs in an ABA-dependent manner, whereas most of the subgroup II and III GmPYLs bind to PP2Cs in an ABA-independent manner. The subgroup III GmPYL23, which cannot interact with any of the tested PP2Cs, differs from other GmPYLs. The CL2/gate domain is crucial for GmPYLs-PP2Cs interaction, and a mutation in the conserved proline (P109S) abolishes the interaction between GmPYL1 and AtABI1. Furthermore, the ABA dependence of GmPYLs-PP2Cs interactions are partially correlated with two amino acid residues preceding the CL2/gate domain of GmPYLs. We also show that GmPYL1 interacts with AtABI1 in an ABA-dependent manner in plant cells. Three GmPYLs differentially inhibit AtABI1 and GmPP2C1 in an ABA-dependent or -enhanced manner in vitro. In addition, ectopically expressing GmPYL1 partially restores ABA sensitivity of the Arabidopsis triple mutant pyr1/pyl1/pyl4. Taken together, our results suggest that soybean GmPYLs are ABA receptors that function by interacting and inhibiting PP2Cs.

MeSH Terms
Abscisic Acid/metabolism Arabidopsis Proteins/genetics,metabolism Carrier Proteins/genetics,metabolism Cloning, Molecular Intracellular Signaling Peptides and Proteins Membrane Transport Proteins/genetics,metabolism Phosphoprotein Phosphatases/genetics,metabolism Phylogeny Plant Growth Regulators/metabolism Protein Phosphatase 2C Soybeans/genetics,metabolism Two-Hybrid System Techniques
Chemicals
Arabidopsis Proteins Carrier Proteins Intracellular Signaling Peptides and Proteins Membrane Transport Proteins PYL2 protein, Arabidopsis Plant Growth Regulators Pyr1 protein, Arabidopsis RCAR1 protein, Arabidopsis Abscisic Acid Phosphoprotein Phosphatases Protein Phosphatase 2C
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Bai Ge
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P. R. China, 200240. | Shanghai Center for Plant Stress Biology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai, P. R. China, 201602.
Yang Da-Hai
Shanghai Center for Plant Stress Biology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai, P. R. China, 201602. | Institute of Plant Physiology and Ecology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai, P. R. China, 200032.
Zhao Yang
Shanghai Center for Plant Stress Biology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai, P. R. China, 201602. | Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA.
Ha Si
Shanghai Center for Plant Stress Biology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai, P. R. China, 201602. | Institute of Plant Physiology and Ecology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai, P. R. China, 200032.
Yang Fen
Shanghai Center for Plant Stress Biology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai, P. R. China, 201602.
Ma Jun
Shanghai Center for Plant Stress Biology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai, P. R. China, 201602.
Gao Xiao-Su
Institute of Plant Physiology and Ecology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai, P. R. China, 200032.
Wang Zhi-Min
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P. R. China, 200240.
Zhu Jian-Kang
Shanghai Center for Plant Stress Biology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai, P. R. China, 201602. | Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA.
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
1573-5028
DOI
10.1007/s11103-013-0114-4
Published
2013-12-00
Epub
2013-00-10
Pages
651-64
Language
English
Region
Netherlands
NLM ID
9106343
PMCID
PMC3834219
Subset
IM
Grants
NIGMS NIH HHS · R01 GM059138 · United States
External Links
PubMed source DOI: 10.1007/s11103-013-0114-4 PMC Full Text View journal details
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