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PMID: 22829320 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Unique drought resistance functions of the highly ABA-induced clade A protein phosphatase 2Cs.

Plant physiology ·Vol. 160 ·No. 1 ·2012-09-00 ·Pages 379-95

Bhaskara GB, Nguyen TT, Verslues PE

Abstract

Six Arabidopsis (Arabidopsis thaliana) clade A protein phosphatase 2Cs (PP2Cs) have established abscisic acid (ABA) signaling roles; however, phenotypic roles of the remaining three "HAI" PP2Cs, Highly ABA-Induced1 (HAI1), AKT1-Interacting PP2C1/HAI2, and HAI3, have remained unclear. HAI PP2C mutants had enhanced proline and osmoregulatory solute accumulation at low water potential, while mutants of other clade A PP2Cs had no or lesser effect on these drought resistance traits. hai1-2 also had increased expression of abiotic stress-associated genes, including dehydrins and late embryogenesis abundant proteins, but decreased expression of several defense-related genes. Conversely, the HAI PP2Cs had relatively less impact on several ABA sensitivity phenotypes. HAI PP2C single mutants were unaffected in ABA sensitivity, while double and triple mutants were moderately hypersensitive in postgermination ABA response but ABA insensitive in germination. The HAI PP2Cs interacted most strongly with PYL5 and PYL7 to -10 of the PYL/RCAR ABA receptor family, with PYL7 to -10 interactions being relatively little affected by ABA in yeast two-hybrid assays. HAI1 had especially limited PYL interaction. Reduced expression of the main HAI1-interacting PYLs at low water potential when HAI1 expression was strongly induced also suggests limited PYL regulation and a role of HAI1 activity in negatively regulating specific drought resistance phenotypes. Overall, the HAI PP2Cs had greatest effect on ABA-independent low water potential phenotypes and lesser effect on classical ABA sensitivity phenotypes. Both this and their distinct PYL interaction demonstrate a new level of functional differentiation among the clade A PP2Cs and a point of cross talk between ABA-dependent and ABA-independent drought-associated signaling.

MeSH Terms
Abscisic Acid/pharmacology Adaptation, Physiological Arabidopsis/drug effects,enzymology,genetics Arabidopsis Proteins/genetics,metabolism Carrier Proteins/genetics,metabolism Droughts Gene Expression Regulation, Plant Genes, Plant Germination Osmosis Phenotype Phosphoprotein Phosphatases/genetics,metabolism Protein Phosphatase 2C Receptors, Cell Surface/genetics,metabolism Seeds/drug effects Signal Transduction Soil/chemistry Stress, Physiological Two-Hybrid System Techniques Water/physiology
Chemicals
AIP1 protein, Arabidopsis Arabidopsis Proteins Carrier Proteins PYL10 protein, Arabidopsis Receptors, Cell Surface Soil Water Abscisic Acid Phosphoprotein Phosphatases Protein Phosphatase 2C
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bhaskara Govinal Badiger
Institute of Plant and Microbial Biology, Academia Sinica, Taipei, 11529 Taiwan.
Nguyen Thao Thi
Verslues Paul E
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
1532-2548
DOI
10.1104/pp.112.202408
Published
2012-09-00
Epub
2012-00-24
Pages
379-95
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC3440212
Subset
IM
Corrections
ErratumIn
PMID: 30425159
External Links
PubMed source DOI: 10.1104/pp.112.202408 PMC Full Text View journal details
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