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PMID: 11500565 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and characterization of a coronatine-regulated tyrosine aminotransferase from Arabidopsis.

Plant physiology ·Vol. 126 ·No. 4 ·2001-08-00 ·Pages 1678-87

Lopukhina A, Dettenberg M, Weiler EW, Holländer-Czytko H

Abstract

In plants, the phytotoxin coronatine, which is an analog of the octadecanoids 12-oxo-phytodienoic acid and/or jasmonic acid, gives rise to a number of physiological responses similar to those of octadecanoids. To further elucidate the physiological role of these compounds, the differential RNA display technique was used to isolate a number of novel octadecanoid-inducible genes expressed in coronatine-treated Arabidopsis. Among these, a cDNA clone was identified that was similar to known tyrosine aminotransferases (TATs). The function was verified with the expressed recombinant protein. In Arabidopsis, the protein is present as a multimer of 98 kD, with a monomer of an apparent molecular mass of 47 kD. TAT mRNA could be induced within 2 h by various octadecanoids and by wounding of the plants. Accumulation of the TAT protein and a 5- to 7-fold increase in its enzymatic activity was observed 7 to 9 h after application of octadecanoids, coronatine, or wounding. The potential role of TAT in the defense response to herbivores and pathogens is discussed.

MeSH Terms
Amino Acid Sequence Amino Acids/physiology Animals Arabidopsis/drug effects,enzymology,genetics Cloning, Molecular DNA, Complementary DNA, Plant Gene Expression Profiling Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Humans Indenes Molecular Sequence Data Plant Diseases RNA, Messenger RNA, Plant Stearic Acids/metabolism,pharmacology Tyrosine Transaminase/genetics,isolation & purification,metabolism
Chemicals
Amino Acids DNA, Complementary DNA, Plant Indenes RNA, Messenger RNA, Plant Stearic Acids coronatine Tyrosine Transaminase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lopukhina A
Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität, D-44780 Bochum, Germany.
Dettenberg M
Weiler E W
Holländer-Czytko H
References (48)
48 references, click to expand
  1. Antioxidant activity of reduced plastoquinone in chloroplast thylakoid membranes.
    Arch Biochem Biophys. 1995 Dec 1;324(1):117-22 PMID: 7503545
  2. Jasmonic acid-dependent and -independent signaling pathways control wound-induced gene activation in Arabidopsis thaliana.
    Plant Physiol. 1997 Oct;115(2):817-26 PMID: 9342878
  3. Two Methyl Jasmonate-Insensitive Mutants Show Altered Expression of AtVsp in Response to Methyl Jasmonate and Wounding.
    Plant Physiol. 1996 Jun;111(2):525-531 PMID: 12226307
  4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  5. Using CLUSTAL for multiple sequence alignments.
    Methods Enzymol. 1996;266:383-402 PMID: 8743695
  6. Arabidopsis Mutants Selected for Resistance to the Phytotoxin Coronatine Are Male Sterile, Insensitive to Methyl Jasmonate, and Resistant to a Bacterial Pathogen.
    Plant Cell. 1994 May;6(5):751-759 PMID: 12244256
  7. Differential expression of tomato proteinase inhibitor I and II genes during bacterial pathogen invasion and wounding.
    Mol Plant Microbe Interact. 1991 May-Jun;4(3):284-92 PMID: 1932815
  8. The structure of tyrosine aminotransferase. Evidence for domains involved in catalysis and enzyme turnover.
    J Biol Chem. 1989 Jan 5;264(1):45-53 PMID: 2562840
  9. Jasmonate is essential for insect defense in Arabidopsis.
    Proc Natl Acad Sci U S A. 1997 May 13;94(10):5473-7 PMID: 11038546
  10. Allene oxide synthase: a major control point in Arabidopsis thaliana octadecanoid signalling.
    Plant J. 1998 Sep;15(5):675-84 PMID: 9778849
  11. Aspartate aminotransferase activity is required for aspartate catabolism and symbiotic nitrogen fixation in Rhizobium meliloti.
    J Bacteriol. 1991 May;173(9):2879-87 PMID: 2019560
  12. Characterization and subcellular compartmentation of recombinant 4-hydroxyphenylpyruvate dioxygenase from Arabidopsis in transgenic tobacco.
    Plant Physiol. 1999 Apr;119(4):1507-16 PMID: 10198110
  13. Isolation of cDNA clones for genes showing enhanced expression in barley leaves during dark-induced senescence as well as during senescence under field conditions.
    Planta. 1997;203(3):332-40 PMID: 9431680
  14. Differential expression of a novel gene in response to coronatine, methyl jasmonate, and wounding in the Coi1 mutant of Arabidopsis.
    Plant Physiol. 1998 Mar;116(3):1037-42 PMID: 9501136
  15. A role for jasmonate in pathogen defense of Arabidopsis.
    Proc Natl Acad Sci U S A. 1998 Jun 9;95(12):7209-14 PMID: 9618564
  16. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  17. Role of the phytotoxin coronatine in the infection of Arabidopsis thaliana by Pseudomonas syringae pv. tomato.
    Mol Plant Microbe Interact. 1995 Jan-Feb;8(1):165-71 PMID: 7539639
  18. SA, JA, ethylene, and disease resistance in plants.
    Curr Opin Plant Biol. 1998 Aug;1(4):316-23 PMID: 10066607
  19. Tissue-dependent plastid RNA splicing in maize: transcripts from four plastid genes are predominantly unspliced in leaf meristems and roots.
    Plant Cell. 1989 Apr;1(4):437-45 PMID: 2562564
  20. Localization and synthesis of prenylquinones in isolated outer and inner envelope membranes from spinach chloroplasts.
    Arch Biochem Biophys. 1985 Apr;238(1):290-9 PMID: 3985624
  21. Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form.
    Anal Biochem. 1991 Dec;199(2):223-31 PMID: 1812789
  22. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction.
    Science. 1992 Aug 14;257(5072):967-71 PMID: 1354393
  23. Wounding and chemicals induce expression of the Arabidopsis thaliana gene Thi2.1, encoding a fungal defense thionin, via the octadecanoid pathway.
    FEBS Lett. 1998 Oct 23;437(3):281-6 PMID: 9824308
  24. Basic local alignment search tool.
    J Mol Biol. 1990 Oct 5;215(3):403-10 PMID: 2231712
  25. Complementation of the Arabidopsis pds1 mutation with the gene encoding p-hydroxyphenylpyruvate dioxygenase.
    Plant Physiol. 1998 Aug;117(4):1317-23 PMID: 9701587
  26. Cloning, molecular and functional characterization of Arabidopsis thaliana allene oxide synthase (CYP 74), the first enzyme of the octadecanoid pathway to jasmonates.
    Plant Mol Biol. 1996 May;31(2):323-35 PMID: 8756596
  27. Dexamethasone phosphate-induced synthesis of tyrosine aminotransferase in hepatoma tissue culture cells. Studies of the early phases of induction and of the steroid requirement for maintanance of the induced rate of synthesis.
    J Biol Chem. 1970 Mar 25;245(6):1472-8 PMID: 4392629
  28. Induction of Arabidopsis defense genes by virulent and avirulent Pseudomonas syringae strains and by a cloned avirulence gene.
    Plant Cell. 1991 Jan;3(1):61-72 PMID: 1824335
  29. Geranyl diphosphate synthase: cloning, expression, and characterization of this prenyltransferase as a heterodimer.
    Proc Natl Acad Sci U S A. 1999 Nov 9;96(23):13062-7 PMID: 10557273
  30. Isolation and characterization of a gene from Trypanosoma cruzi encoding a 46-kilodalton protein with homology to human and rat tyrosine aminotransferase.
    Mol Biochem Parasitol. 1993 Jun;59(2):253-62 PMID: 8101971
  31. Isolation and characterization of the human tyrosine aminotransferase gene.
    Nucleic Acids Res. 1990 Jul 11;18(13):3853-61 PMID: 1973834
  32. Coi1-dependent expression of an Arabidopsis vegetative storage protein in flowers and siliques and in response to coronatine or methyl jasmonate.
    Plant Physiol. 1995 Oct;109(2):567-72 PMID: 7480347
  33. Protective action of seven natural phenolic compounds against peroxidative damage to biomembranes.
    Biochem Pharmacol. 1992 Jan 22;43(2):147-52 PMID: 1739402
  34. Rapid PCR-based method to directionally pull out longer cDNA fragments from cDNA libraries.
    Biotechniques. 1995 Jan;18(1):36, 38, 40 PMID: 7702849
  35. The Pseudomonas phytotoxin coronatine mimics octadecanoid signalling molecules of higher plants.
    FEBS Lett. 1994 May 23;345(1):9-13 PMID: 8194607
  36. Midiprep method for isolation of DNA from plants with a high content of polyphenolics.
    Nucleic Acids Res. 1993 Jul 11;21(14):3328 PMID: 8341613
  37. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  38. The wound response of plants.
    Curr Biol. 1991 Jun;1(3):165-7 PMID: 15336155
  39. Elevating the vitamin E content of plants through metabolic engineering.
    Science. 1998 Dec 11;282(5396):2098-100 PMID: 9851934
  40. The formation of homogentisate in the biosynthesis of tocopherol and plastoquinone in spinach chloroplasts.
    Planta. 1982 Nov;155(6):511-5 PMID: 24272117
  41. Biosynthesis of jasmonic Acid by several plant species.
    Plant Physiol. 1984 Jun;75(2):458-61 PMID: 16663643
  42. Induction of 12-oxo-phytodienoic acid in wounded plants and elicited plant cell cultures.
    Plant Physiol. 1997 Nov;115(3):1057-64 PMID: 9390438
  43. PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization.
    Trends Biochem Sci. 1999 Jan;24(1):34-6 PMID: 10087920
  44. Interplant communication: airborne methyl jasmonate induces synthesis of proteinase inhibitors in plant leaves.
    Proc Natl Acad Sci U S A. 1990 Oct;87(19):7713-6 PMID: 11607107
  45. Subcellular localization and purification of a p-hydroxyphenylpyruvate dioxygenase from cultured carrot cells and characterization of the corresponding cDNA.
    Biochem J. 1997 Aug 1;325 ( Pt 3):761-9 PMID: 9271098
  46. Conversion of 4-hydroxyphenylpyruvic acid into homogentisic acid at the thylakoid membrane of Lemna gibba.
    FEBS Lett. 1979 Aug 15;104(2):332-4 PMID: 477996
  47. Purification and characterization of tyrosine aminotransferase activities from Anchusa officinalis cell cultures.
    Arch Biochem Biophys. 1987 Sep;257(2):430-8 PMID: 2889425
  48. Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures.
    Proc Natl Acad Sci U S A. 1992 Mar 15;89(6):2389-93 PMID: 11607285
Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.126.4.1678
Published
2001-08-00
Pages
1678-87
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC117166
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.126.4.1678 PMC Full Text View journal details
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