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PMID: 16126835 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Secretome analysis reveals an Arabidopsis lipase involved in defense against Alternaria brassicicola.

The Plant cell ·Vol. 17 ·No. 10 ·2005-10-00 ·Pages 2832-47

Oh IS, Park AR, Bae MS, Kwon SJ, Kim YS, Lee JE, Kang NY, Lee S, Cheong H, Park OK

Abstract

The Arabidopsis thaliana secretome was analyzed by the proteomic approach, which led to the identification of secreted proteins implicated in many aspects of cell biology. We then investigated the change in the Arabidopsis secretome in response to salicylic acid and identified several proteins involved in pathogen response. One of these, a secreted lipase with a GDSL-like motif designated GDSL LIPASE1 (GLIP1), was further characterized for its function in disease resistance. glip1 plants were markedly more susceptible to infection by the necrotrophic fungus Alternaria brassicicola compared with the parental wild-type plants. The recombinant GLIP1 protein possessed lipase and antimicrobial activities that directly disrupt fungal spore integrity. Furthermore, GLIP1 appeared to trigger systemic resistance signaling in plants when challenged with A. brassicicola, because pretreatment of the glip1 mutant with recombinant GLIP1 protein inhibited A. brassicicola-induced cell death in both peripheral and distal leaves. Moreover, glip1 showed altered expression of defense- and ethylene-related genes. GLIP1 transcription was increased by ethephon, the ethylene releaser, but not by salicylic acid or jasmonic acid. These results suggest that GLIP1, in association with ethylene signaling, may be a critical component in plant resistance to A. brassicicola.

MeSH Terms
Alternaria/physiology Amino Acid Motifs/genetics Arabidopsis/enzymology,genetics Arabidopsis Proteins/genetics,isolation & purification,metabolism Carboxylic Ester Hydrolases/genetics,isolation & purification,metabolism Cells, Cultured Conserved Sequence/genetics Gene Expression Regulation, Plant/genetics Genetic Predisposition to Disease/genetics Immunity, Innate/genetics Lipase/genetics,isolation & purification,metabolism Molecular Sequence Data Mutation/genetics Organophosphorus Compounds/pharmacology Plant Diseases/genetics Proteomics Recombinant Fusion Proteins/genetics,metabolism,pharmacology Salicylic Acid/pharmacology Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Transcriptional Activation/drug effects,genetics
Chemicals
Arabidopsis Proteins Organophosphorus Compounds Recombinant Fusion Proteins Carboxylic Ester Hydrolases Glip1 protein, Arabidopsis Lipase Salicylic Acid ethephon
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Oh Il Seok
School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.
Park Ae Ran
Bae Min Seok
Kwon Sun Jae
Kim Young Soon
Lee Ji Eun
Kang Na Young
Lee Sumin
Cheong Hyeonsook
Park Ohkmae K
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.105.034819
Published
2005-10-00
Epub
2005-00-26
Pages
2832-47
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1242276
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.105.034819 PMC Full Text View journal details
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