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PMID: 8989885 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Pathogen-induced systemic activation of a plant defensin gene in Arabidopsis follows a salicylic acid-independent pathway.

The Plant cell ·Vol. 8 ·No. 12 ·1996-12-00 ·Pages 2309-23

Penninckx IA, Eggermont K, Terras FR, Thomma BP, De Samblanx GW, Buchala A, Métraux JP, Manners JM, Broekaert WF

Abstract

A 5-kD plant defensin was purified from Arabidopsis leaves challenged with the fungus Alternaria brassicicola and shown to possess antifungal properties in vitro. The corresponding plant defensin gene was induced after treatment of leaves with methyl jasmonate or ethylene but not with salicylic acid or 2,6-dichloroisonicotinic acid. When challenged with A. brassicicola, the levels of the plant defensin protein and mRNA rose both in inoculated leaves and in nontreated leaves of inoculated plants (systemic leaves). These events coincided with an increase in the endogenous jasmonic acid content of both types of leaves. Systemic pathogen-induced expression of the plant defensin gene was unaffected in Arabidopsis transformants (nahG) or mutants (npr1 and cpr1) affected in the salicylic acid response but was strongly reduced in the Arabidopsis mutants eln2 and col1 that are blocked in their response to ethylene and methyl jasmonate, respectively. Our results indicate that systemic pathogen-induced expression of the plant defensin gene in Arabidopsis is independent of salicylic acid but requires components of the ethylene and jasmonic acid response.

MeSH Terms
Alternaria/pathogenicity Amino Acid Sequence Antifungal Agents Arabidopsis/microbiology,physiology Base Sequence Cyclopentanes/metabolism Defensins Gene Expression Regulation, Plant Genes, Plant Kinetics Molecular Sequence Data Mutagenesis Oxylipins Plant Leaves Plant Proteins/biosynthesis,chemistry,isolation & purification Polymerase Chain Reaction Protein Biosynthesis RNA, Messenger/biosynthesis Sequence Homology, Amino Acid Transcription, Genetic
Chemicals
Antifungal Agents Cyclopentanes Defensins Oxylipins Plant Proteins RNA, Messenger jasmonic acid
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Penninckx I A
F. A. Janssens Laboratory of Genetics, Katholieke Universiteit Leuven, Heverlee, Belgium.
Eggermont K
Terras F R
Thomma B P
De Samblanx G W
Buchala A
Métraux J P
Manners J M
Broekaert W F
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.8.12.2309
Published
1996-12-00
Pages
2309-23
Language
English
Region
England
NLM ID
9208688
PMCID
PMC161354
Subset
IM
Databases
GENBANK
T04323, Z27258
External Links
PubMed source DOI: 10.1105/tpc.8.12.2309 PMC Full Text View journal details
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