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PMID: 12242373 Published · ppublish English Journal Article

UV-B-Induced PR-1 Accumulation Is Mediated by Active Oxygen Species.

The Plant cell ·Vol. 7 ·No. 2 ·1995-02-00 ·Pages 203-212

Green R, Fluhr R

Abstract

Depletion of the stratospheric ozone layer may result in an increase in the levels of potentially harmful UV-B radiation reaching the surface of the earth. We have found that UV-B is a potent inducer of the plant pathogenesis-related protein PR-1 in tobacco leaves. UV-B fluences required for PR-1 accumulation are similar to those of other UV-B-induced responses. The UV-B-induced PR-1 accumulation was confined precisely to the irradiated area of the leaf but displayed no leaf tissue specificity. A study of some of the possible components of the signal transduction pathway between UV-B and PR-1 induction showed that photosynthetic processes are not essential, and photoreversible DNA damage is not involved. Antioxidants and cycloheximide were able to block the induction of PR-1 by UV-B, and treatment of leaves with a generator of reactive oxygen resulted in the accumulation of PR-1 protein. These results demonstrate an absolute requirement for active oxygen species and protein synthesis in this UV-B signal transduction pathway. In contrast, we also show that other elicitors, notably salicylic acid, are able to elicit PR-1 via nonreactive oxygen species-requiring pathways.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Green R.
Department of Plant Genetics, Weizmann Institute of Science, Rehovot, 76100, Israel.
Fluhr R.
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.7.2.203
Published
1995-02-00
Pages
203-212
Language
English
Region
England
NLM ID
9208688
PMCID
PMC160776
External Links
PubMed source DOI: 10.1105/tpc.7.2.203 PMC Full Text View journal details
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