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PMID: 24179127 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

ABI1 and PP2CA phosphatases are negative regulators of Snf1-related protein kinase1 signaling in Arabidopsis.

The Plant cell ·Vol. 25 ·No. 10 ·2013-10-00 ·Pages 3871-84

Rodrigues A, Adamo M, Crozet P, Margalha L, Confraria A, Martinho C, Elias A, Rabissi A, Lumbreras V, González-Guzmán M, Antoni R, Rodriguez PL, Baena-González E

Abstract

Plant survival under environmental stress requires the integration of multiple signaling pathways into a coordinated response, but the molecular mechanisms underlying this integration are poorly understood. Stress-derived energy deprivation activates the Snf1-related protein kinases1 (SnRK1s), triggering a vast transcriptional and metabolic reprogramming that restores homeostasis and promotes tolerance to adverse conditions. Here, we show that two clade A type 2C protein phosphatases (PP2Cs), established repressors of the abscisic acid (ABA) hormonal pathway, interact with the SnRK1 catalytic subunit causing its dephosphorylation and inactivation. Accordingly, SnRK1 repression is abrogated in double and quadruple pp2c knockout mutants, provoking, similarly to SnRK1 overexpression, sugar hypersensitivity during early seedling development. Reporter gene assays and SnRK1 target gene expression analyses further demonstrate that PP2C inhibition by ABA results in SnRK1 activation, promoting SnRK1 signaling during stress and once the energy deficit subsides. Consistent with this, SnRK1 and ABA induce largely overlapping transcriptional responses. Hence, the PP2C hub allows the coordinated activation of ABA and energy signaling, strengthening the stress response through the cooperation of two key and complementary pathways.

MeSH Terms
Abscisic Acid/metabolism Arabidopsis/genetics,metabolism Arabidopsis Proteins/genetics,metabolism Gene Expression Regulation, Plant Phosphoprotein Phosphatases/genetics,metabolism Phosphorylation Plant Growth Regulators/metabolism Protein Serine-Threonine Kinases/genetics,metabolism Signal Transduction Stress, Physiological
Chemicals
Arabidopsis Proteins Plant Growth Regulators Abscisic Acid Protein Serine-Threonine Kinases SnRK1 protein, Arabidopsis ABI1 protein, Arabidopsis AHG3 protein, Arabidopsis Phosphoprotein Phosphatases
Authors & Affiliations
13 authors, click to expand affiliations / ORCID
Rodrigues Américo
Instituto Gulbenkian de Ciência, 2780-156 Oeiras, Portugal.
Adamo Mattia
Crozet Pierre
Margalha Leonor
Confraria Ana
Martinho Cláudia
Elias Alexandre
Rabissi Agnese
Lumbreras Victoria
González-Guzmán Miguel
Antoni Regina
Rodriguez Pedro L
Baena-González Elena
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.113.114066
Published
2013-10-00
Epub
2013-00-31
Pages
3871-84
Language
English
Region
England
NLM ID
9208688
PMCID
PMC3877788
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.113.114066 PMC Full Text View journal details
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