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PMID: 17075132 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Solution structure of the kinase-associated domain 1 of mouse microtubule-associated protein/microtubule affinity-regulating kinase 3.

Protein science : a publication of the Protein Society ·Vol. 15 ·No. 11 ·2006-11-00 ·Pages 2534-43

Tochio N, Koshiba S, Kobayashi N, Inoue M, Yabuki T, Aoki M, Seki E, Matsuda T, Tomo Y, Motoda Y, Kobayashi A, Tanaka A, Hayashizaki Y, Terada T, Shirouzu M, Kigawa T, Yokoyama S

Abstract

Microtubule-associated protein/microtubule affinity-regulating kinases (MARKs)/PAR-1 are common regulators of cell polarity that are conserved from nematode to human. All of these kinases have a highly conserved C-terminal domain, which is termed the kinase-associated domain 1 (KA1), although its function is unknown. In this study, we determined the solution structure of the KA1 domain of mouse MARK3 by NMR spectroscopy. We found that approximately 50 additional residues preceding the previously defined KA1 domain are required for its proper folding. The newly defined KA1 domain adopts a compact alpha+beta structure with a betaalphabetabetabetabetaalpha topology. We also found a characteristic hydrophobic, concave surface surrounded by positively charged residues. This concave surface includes the highly conserved Glu-Leu-Lys-Leu motif at the C terminus, indicating that it is important for the function of the KA1 domain.

MeSH Terms
Amino Acid Sequence Animals Binding Sites Hydrophobic and Hydrophilic Interactions Mice Microtubule-Associated Proteins/chemistry Models, Molecular Molecular Sequence Data Nuclear Magnetic Resonance, Biomolecular/methods Protein Folding Protein Serine-Threonine Kinases/chemistry Protein Structure, Tertiary Sequence Alignment/methods Sequence Homology, Amino Acid Solvents Structural Homology, Protein
Chemicals
Microtubule-Associated Proteins Solvents MARK3 protein, human Protein Serine-Threonine Kinases
Authors & Affiliations
17 authors, click to expand affiliations / ORCID
Tochio Naoya
RIKEN Genomic Sciences Center, Tsurumi, Yokohama 230-0045, Japan.
Koshiba Seizo
Kobayashi Naohiro
Inoue Makoto
Yabuki Takashi
Aoki Masaaki
Seki Eiko
Matsuda Takayoshi
Tomo Yasuko
Motoda Yoko
Kobayashi Atsuo
Tanaka Akiko
Hayashizaki Yoshihide
Terada Takaho
Shirouzu Mikako
Kigawa Takanori
Yokoyama Shigeyuki
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Article Info
Journal
Protein science : a publication of the Protein Society
Abbr.
Protein Sci
ISSN
0961-8368
DOI
10.1110/ps.062391106
Published
2006-11-00
Pages
2534-43
Language
English
Region
United States
NLM ID
9211750
PMCID
PMC2242405
Subset
IM
External Links
PubMed source DOI: 10.1110/ps.062391106 PMC Full Text View journal details
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