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PMID: 24591651 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Review

Delineating the structural blueprint of the pre-mRNA 3'-end processing machinery.

Molecular and cellular biology ·Vol. 34 ·No. 11 ·2014-06-00 ·Pages 1894-910

Xiang K, Tong L, Manley JL

Abstract

Processing of mRNA precursors (pre-mRNAs) by polyadenylation is an essential step in gene expression. Polyadenylation consists of two steps, cleavage and poly(A) synthesis, and requires multiple cis elements in the pre-mRNA and a megadalton protein complex bearing the two essential enzymatic activities. While genetic and biochemical studies remain the major approaches in characterizing these factors, structural biology has emerged during the past decade to help understand the molecular assembly and mechanistic details of the process. With structural information about more proteins and higher-order complexes becoming available, we are coming closer to obtaining a structural blueprint of the polyadenylation machinery that explains both how this complex functions and how it is regulated and connected to other cellular processes.

MeSH Terms
Animals Cleavage And Polyadenylation Specificity Factor/chemistry,genetics Cleavage Stimulation Factor/chemistry,genetics Gene Expression Humans Poly A/genetics,metabolism Polyadenylation/genetics Protein Structure, Tertiary RNA 3' End Processing/genetics RNA Precursors/genetics,metabolism RNA, Messenger/genetics,metabolism
Chemicals
Cleavage And Polyadenylation Specificity Factor Cleavage Stimulation Factor RNA Precursors RNA, Messenger Poly A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Xiang Kehui
Department of Biological Sciences, Columbia University, New York, New York, USA.
Tong Liang
Manley James L
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
1098-5549
DOI
10.1128/MCB.00084-14
Published
2014-06-00
Epub
2014-00-03
Pages
1894-910
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC4019069
Subset
IM
Grants
NIGMS NIH HHS · R01 GM028983 · United States
NIGMS NIH HHS · R01 GM077175 · United States
NIGMS NIH HHS · GM077175 · United States
NIGMS NIH HHS · GM28983 · United States
External Links
PubMed source DOI: 10.1128/MCB.00084-14 PMC Full Text View journal details
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