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PMID: 2455815 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Immunological characterization of proteins detected by phosphotyrosine antibodies in cells transformed by Rous sarcoma virus.

Journal of virology ·Vol. 62 ·No. 8 ·1988-08-00 ·Pages 2665-73

Linder ME, Burr JG

Abstract

Phosphotyrosine antibodies were used to identify tyrosine-phosphorylated proteins in Rous sarcoma virus (RSV)-transformed chicken embryo fibroblasts. A large number of tyrosine phosphoproteins were detected. A similar set of proteins was observed in RSV-transformed murine cells. An 85,000-dalton protein, however, was present in transformed avian cells but missing in transformed murine cells. Neither the 85,000-dalton protein nor any of the other tyrosine phosphoproteins appeared to be viral structural proteins. Use of RSV mutants encoding partially deleted src gene products enabled us to identify a 60,000-dalton cellular tyrosine phosphoprotein that comigrated with wild-type pp60v-src. With the exception of calpactin I, the major tyrosine phosphoproteins detected in immunoblots appeared to be different from several previously characterized substrates of pp60v-src with similar molecular masses (ezrin, vinculin, and the fibronectin receptor).

MeSH Terms
Annexins Avian Sarcoma Viruses Calcium-Binding Proteins/metabolism Cell Line Cell Transformation, Viral Cytoskeletal Proteins Molecular Weight Muscle Proteins/metabolism Oncogene Protein pp60(v-src) Phosphoproteins/immunology,metabolism Phosphotyrosine Receptors, Fibronectin Receptors, Immunologic/metabolism Retroviridae Proteins/metabolism Tyrosine/analogs & derivatives,immunology Vinculin
Chemicals
Annexins Calcium-Binding Proteins Cytoskeletal Proteins Muscle Proteins Phosphoproteins Receptors, Fibronectin Receptors, Immunologic Retroviridae Proteins ezrin Vinculin Phosphotyrosine Tyrosine Oncogene Protein pp60(v-src)
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Linder M E
Program in Molecular Biology, University of Texas, Dallas, Richardson 75083-0688.
Burr J G
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57 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.62.8.2665-2673.1988
Published
1988-08-00
Pages
2665-73
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253698
Subset
IM
Grants
DRS NIH HHS · BRSG 2507 · United States
External Links
PubMed source DOI: 10.1128/JVI.62.8.2665-2673.1988 PMC Full Text View journal details
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