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PMID: 6199661 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Diverse mitogenic agents induce the phosphorylation of two related 42,000-dalton proteins on tyrosine in quiescent chick cells.

Molecular and cellular biology ·Vol. 4 ·No. 1 ·1984-01-00 ·Pages 30-7

Cooper JA, Sefton BM, Hunter T

Abstract

Incubation of quiescent chicken embryo cells with platelet-derived growth factor, epidermal growth factor, or serum was found to stimulate phosphorylation of two proteins of ca. 42,000 daltons on tyrosine. These proteins are structurally related to each other and to two proteins phosphorylated on tyrosine under similar conditions in mitogen-treated mouse fibroblasts. Three other very different mitogenic agents, the protease trypsin and the chemically unrelated tumor promoters 12-O-tetradecanoyl-phorbol-13-acetate and teleocidin, stimulated phosphorylation of the same proteins. In all cases, phosphotyrosine was detected in these phosphoproteins. Although additional changes in protein phosphorylation were evident, no other proteins were observed by two-dimensional gel electrophoresis which contained increased amounts of phosphotyrosine in mitogen-treated chicken embryo cells. One of these 42,000-dalton proteins was shown previously to be phosphorylated on tyrosine in chicken embryo cells transformed with various retroviruses whose transforming proteins possess tyrosine protein kinase activity. Phosphorylation of the 42,000-dalton proteins could be important in the regulation of cell division.

MeSH Terms
Animals Cell Division Cells, Cultured Chick Embryo Mice Mitogens/pharmacology Molecular Weight Phosphorylation Phosphotyrosine Protein Kinases/metabolism Proteins/metabolism Tyrosine/analogs & derivatives,biosynthesis,metabolism
Chemicals
Mitogens Proteins Phosphotyrosine Tyrosine Protein Kinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cooper J A
Sefton B M
Hunter T
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62 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.1.30-37.1984
Published
1984-01-00
Pages
30-7
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368654
Subset
IM
Grants
NCI NIH HHS · CA 14195 · United States
NCI NIH HHS · CA 17096 · United States
NCI NIH HHS · CA 17289 · United States
External Links
PubMed source DOI: 10.1128/mcb.4.1.30-37.1984 PMC Full Text View journal details
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