Home LiteratureArticle Details
PMID: 2454397 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Substitution of Ser-17 of pp60c-src: biological and biochemical characterization in chicken embryo fibroblasts.

Molecular and cellular biology ·Vol. 8 ·No. 4 ·1988-04-00 ·Pages 1826-30

Hirota Y, Kato J, Takeya T

Abstract

pp60c-src is phosphorylated mainly on Ser-17 and Tyr-527 in vivo. In this study, we examined the effect of the phosphorylation of Ser-17 on the properties of pp60c-src by introducing Rous sarcoma virus variants carrying pp60c-src in which Ser-17 had been substituted, into chicken embryo fibroblasts. The Ala-17 substitution in wild-type pp60c-src and pp60c-src carrying Phe-527 caused a two- to threefold elevation in the kinase activity in vitro of these proteins; the former variant resulted in no morphological changes of infected cells, whereas the latter variant transformed chicken embryo fibroblasts. Since the substitution of Tyr-527 per se has been reported to activate pp60c-src, these results suggest that the abolishment of the phosphorylation of Ser-17 does not affect noticeably the properties of pp60c-src in chicken embryo fibroblasts.

MeSH Terms
Animals Cells, Cultured Chick Embryo Fibroblasts/cytology,enzymology Genes Genetic Variation Mutation Protein Kinases/genetics Proto-Oncogene Proteins/genetics,metabolism Proto-Oncogene Proteins pp60(c-src) Proto-Oncogenes Serine
Chemicals
Proto-Oncogene Proteins Serine Protein Kinases Proto-Oncogene Proteins pp60(c-src)
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hirota Y
Institute for Chemical Research, Kyoto University, Japan.
Kato J
Takeya T
References (33)
33 references, click to expand
  1. Rapid transformation of cells by Rous sarcoma virus.
    Proc Natl Acad Sci U S A. 1969 Jun;63(2):318-25 PMID: 4308272
  2. Tyr527 is phosphorylated in pp60c-src: implications for regulation.
    Science. 1986 Mar 21;231(4744):1431-4 PMID: 2420005
  3. Structural analysis of the avian sarcoma virus transforming protein: sites of phosphorylation.
    J Virol. 1979 Feb;29(2):770-81 PMID: 219258
  4. Two types of amino acid substitutions in protein evolution.
    J Mol Evol. 1979 Mar 15;12(3):219-36 PMID: 439147
  5. Transforming gene product of Rous sarcoma virus phosphorylates tyrosine.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1311-5 PMID: 6246487
  6. Viral and cellular src genes contribute to the structure of recovered avian sarcoma virus transforming protein.
    Cell. 1981 Apr;24(1):155-64 PMID: 6263484
  7. Characterization of sites for tyrosine phosphorylation in the transforming protein of Rous sarcoma virus (pp60v-src) and its normal cellular homologue (pp60c-src).
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6013-7 PMID: 6273838
  8. DNA sequence of the viral and cellular src gene of chickens. 1. Complete nucleotide sequence of an EcoRI fragment of recovered avian sarcoma virus which codes for gp37 and pp60src.
    J Virol. 1982 Oct;44(1):1-11 PMID: 6292477
  9. DNA sequence of the viral and cellular src gene of chickens. II. Comparison of the src genes of two strains of avian sarcoma virus and of the cellular homolog.
    J Virol. 1982 Oct;44(1):12-8 PMID: 6292480
  10. Corrections to the nucleotide sequence of the src gene of Rous sarcoma virus.
    Nature. 1983 Feb 24;301(5902):736-8 PMID: 6298633
  11. Nucleotide sequence of Rous sarcoma virus.
    Cell. 1983 Mar;32(3):853-69 PMID: 6299578
  12. Activation of the transforming potential of p60c-src by a single amino acid change.
    Proc Natl Acad Sci U S A. 1986 Jun;83(12):4228-32 PMID: 2424022
  13. Restriction of the in vitro and in vivo tyrosine protein kinase activities of pp60c-src relative to pp60v-src.
    Mol Cell Biol. 1985 Oct;5(10):2753-63 PMID: 2426575
  14. Amino acid substitutions sufficient to convert the nontransforming p60c-src protein to a transforming protein.
    Mol Cell Biol. 1986 Dec;6(12):4155-60 PMID: 2432397
  15. Dephosphorylation or antibody binding to the carboxy terminus stimulates pp60c-src.
    Mol Cell Biol. 1986 Dec;6(12):4467-77 PMID: 2432403
  16. Activation and suppression of pp60c-src transforming ability by mutation of its primary sites of tyrosine phosphorylation.
    Cell. 1987 Apr 10;49(1):65-73 PMID: 3103925
  17. Tyrosine phosphorylation regulates the biochemical and biological properties of pp60c-src.
    Cell. 1987 Apr 10;49(1):75-82 PMID: 3103926
  18. Cell transformation by pp60c-src mutated in the carboxy-terminal regulatory domain.
    Cell. 1987 Apr 10;49(1):83-91 PMID: 3103927
  19. Activation of the oncogenic potential of the avian cellular src protein by specific structural alteration of the carboxy terminus.
    EMBO J. 1987 Aug;6(8):2359-64 PMID: 2822389
  20. Changes in protein phosphorylation in Rous sarcoma virus-transformed chicken embryo cells.
    Mol Cell Biol. 1981 Feb;1(2):165-78 PMID: 6100962
  21. Structural features of the carboxy terminus of p60c-src that are required for the regulation of its intrinsic kinase activity.
    Jpn J Cancer Res. 1987 Dec;78(12):1354-62 PMID: 3123438
  22. Structure and sequence of the cellular gene homologous to the RSV src gene and the mechanism for generating the transforming virus.
    Cell. 1983 Mar;32(3):881-90 PMID: 6299580
  23. Local mutagenesis of Rous sarcoma virus: the major sites of tyrosine and serine phosphorylation of pp60src are dispensable for transformation.
    Cell. 1983 Sep;34(2):597-607 PMID: 6311433
  24. Rous sarcoma virus variants that carry the cellular src gene instead of the viral src gene cannot transform chicken embryo fibroblasts.
    Proc Natl Acad Sci U S A. 1984 Jul;81(14):4424-8 PMID: 6087323
  25. pp60c-src is a substrate for phosphorylation when cells are stimulated to enter cycle.
    FEBS Lett. 1984 Nov 5;177(1):151-6 PMID: 6437865
  26. Low level of cellular protein phosphorylation by nontransforming overproduced p60c-src.
    Mol Cell Biol. 1985 May;5(5):1058-66 PMID: 2582236
  27. Tyrosine phosphorylation within the amino-terminal domain of pp60c-src molecules associated with polyoma virus middle-sized tumor antigen.
    Proc Natl Acad Sci U S A. 1985 Jul;82(14):4568-72 PMID: 3927285
  28. Protein-tyrosine kinases.
    Annu Rev Biochem. 1985;54:897-930 PMID: 2992362
  29. Activation of the pp60c-src kinase by middle T antigen binding or by dephosphorylation.
    EMBO J. 1985 Jun;4(6):1471-7 PMID: 2411538
  30. Site-specific increased phosphorylation of pp60v-src after treatment of RSV-transformed cells with a tumor promoter.
    Science. 1985 Sep 27;229(4720):1393-5 PMID: 2994221
  31. Protein kinase C phosphorylates pp60src at a novel site.
    Cell. 1985 Oct;42(3):849-57 PMID: 2996780
  32. The product of the protooncogene c-src is modified during the cellular response to platelet-derived growth factor.
    Proc Natl Acad Sci U S A. 1985 Dec;82(23):7845-9 PMID: 2415973
  33. Characterization of Rous sarcoma virus src gene products synthesized in vitro.
    J Virol. 1978 Nov;28(2):551-66 PMID: 214578
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.4.1826-1830.1988
Published
1988-04-00
Pages
1826-30
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363347
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.8.4.1826-1830.1988 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2