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PMID: 2441081 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of a cDNA clone corresponding to a transcript from the Epstein-Barr virus BamHI M fragment: evidence for overlapping mRNAs.

Journal of virology ·Vol. 61 ·No. 9 ·1987-09-00 ·Pages 2943-6

Pfitzner AJ, Strominger JL, Speck SH

Abstract

A 1.95-kilobase cDNA clone was isolated by screening a size-selected lambda gt10 cDNA library prepared from an Epstein-Barr virus-transformed B-cell line, IB4, with the Epstein-Barr virus BamHI M fragment. Sequence analysis revealed that this clone contains about 75% of the BMRF1 and the complete BMRF2 open reading frames. The transcript is not spliced, and the polyadenylation signal at base pair 2641 of the BamHI M fragment is used. Northern blots (RNA blots) indicate that this polyadenylation signal is used for three overlapping mRNAs. The sizes of these transcripts are 3.5, 2.6, and 1.5 kilobases.

MeSH Terms
Antigens, Viral/genetics Base Sequence DNA/analysis DNA Restriction Enzymes/pharmacology Deoxyribonuclease BamHI Epstein-Barr Virus Nuclear Antigens Herpesvirus 4, Human/genetics Poly A/metabolism RNA/metabolism RNA Splicing RNA, Messenger/analysis Transcription, Genetic
Chemicals
Antigens, Viral Epstein-Barr Virus Nuclear Antigens RNA, Messenger Poly A RNA DNA DNA Restriction Enzymes Deoxyribonuclease BamHI
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pfitzner A J
Strominger J L
Speck S H
References (23)
23 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.9.2943-2946.1987
Published
1987-09-00
Pages
2943-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255831
Subset
IM
Grants
NCI NIH HHS · 1RO1CA43143 · United States
NCI NIH HHS · 5F32CA07147 · United States
NCI NIH HHS · 5PO1CA21082 · United States
Databases
GENBANK
M17322
External Links
PubMed source DOI: 10.1128/JVI.61.9.2943-2946.1987 PMC Full Text View journal details
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