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PMID: 3020506 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Epstein-Barr virus mRNAs produced by alternative splicing.

Nucleic acids research ·Vol. 14 ·No. 17 ·1986-09-11 ·Pages 7103-14

Bodescot M, Perricaudet M

Abstract

The structure of Epstein-Barr virus mRNAs transcribed in B95-8 cells has been studied by cDNA cloning and sequencing. We present here the analysis of four cDNAs. The corresponding mRNAs are probably transcribed from a single promoter located in the US region. They are produced by alternative splicing of exons transcribed from the US, IR and UL regions. The exons are spread over 100 kbp. The exons from the IR region constitute a unit which is repeated several times. The cDNAs share the exons from the US and IR regions. Some of the cDNAs also share some of the exons from the UL region. Each cDNA contains a long open reading frame or the 5' end of a long open reading frame which ends several hundred nucleotides downstream on the viral genome. The 5' untranslated regions are unusually long. Three mRNA species differing in their 5' untranslated regions may encode for the nuclear antigen EBNA-1. The other mRNAs encode for polypeptides which may not have any common region.

MeSH Terms
Cloning, Molecular DNA/genetics Exons Herpesvirus 4, Human/genetics RNA Splicing RNA, Viral/genetics
Chemicals
RNA, Viral DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bodescot M
Perricaudet M
References (37)
37 references, click to expand
  1. Latent and viral replicative transcription in vivo from the BamHI K fragment of Epstein-Barr virus DNA.
    J Virol. 1985 May;54(2):501-8 PMID: 2985812
  2. Nucleotide sequence of murine ornithine decarboxylase mRNA.
    Proc Natl Acad Sci U S A. 1985 Mar;82(6):1673-7 PMID: 3856848
  3. Two major outer envelope glycoproteins of Epstein-Barr virus are encoded by the same gene.
    J Virol. 1985 Jun;54(3):665-74 PMID: 2987520
  4. Sequence-specific DNA binding of the Epstein-Barr virus nuclear antigen (EBNA-1) to clustered sites in the plasmid maintenance region.
    Cell. 1985 Oct;42(3):859-68 PMID: 2996781
  5. An EBV membrane protein expressed in immortalized lymphocytes transforms established rodent cells.
    Cell. 1985 Dec;43(3 Pt 2):831-40 PMID: 3000618
  6. Analysis of the transcript encoding the latent Epstein-Barr virus nuclear antigen I: a potentially polycistronic message generated by long-range splicing of several exons.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8305-9 PMID: 3001694
  7. An Epstein-Barr virus transcription unit is at least 84 kilobases long.
    Nucleic Acids Res. 1986 Mar 25;14(6):2611-20 PMID: 3008094
  8. Epstein-Barr virus: transformation, cytopathic changes, and viral antigens in squirrel monkey and marmoset leukocytes.
    Proc Natl Acad Sci U S A. 1972 Feb;69(2):383-7 PMID: 4333982
  9. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  10. 3' non-coding region sequences in eukaryotic messenger RNA.
    Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
  11. Excision and recombination of adenovirus DNA fragments in Escherichia coli.
    Science. 1977 Apr 8;196(4286):208-10 PMID: 322283
  12. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  13. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  14. In vitro packaging of lambda Dam vectors and their use in cloning DNA fragments.
    Methods Enzymol. 1979;68:281-98 PMID: 232217
  15. Epstein-Barr virus (B95-8) DNA VII: molecular cloning and detailed mapping.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2999-3003 PMID: 6248879
  16. Characterisation of polyoma late mRNA leader sequences by molecular cloning and DNA sequence analysis.
    Nucleic Acids Res. 1980 Nov 11;8(21):4867-88 PMID: 6255435
  17. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  18. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  19. Synthesis and processing of asparagine-linked oligosaccharides.
    Annu Rev Biochem. 1981;50:555-83 PMID: 7023366
  20. Stable expression in mouse cells of nuclear neoantigen after transfer of a 3.4-megadalton cloned fragment of Epstein-Barr virus DNA.
    Proc Natl Acad Sci U S A. 1982 Sep;79(18):5688-92 PMID: 6291059
  21. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  22. Translocation, breakage and truncated transcripts of c-myc oncogene in murine plasmacytomas.
    Nature. 1983 Jun 2-8;303(5916):401-6 PMID: 6304522
  23. Simple repeat array in Epstein-Barr virus DNA encodes part of the Epstein-Barr nuclear antigen.
    Science. 1983 Jun 24;220(4604):1396-8 PMID: 6304878
  24. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5 PMID: 6575390
  25. One of two Epstein-Barr virus nuclear antigens contains a glycine-alanine copolymer domain.
    Proc Natl Acad Sci U S A. 1983 Sep;80(18):5665-9 PMID: 6310587
  26. A simple and very efficient method for generating cDNA libraries.
    Gene. 1983 Nov;25(2-3):263-9 PMID: 6198242
  27. Identification of Epstein-Barr nuclear antigen polypeptide in mouse and monkey cells after gene transfer with a cloned 2.9-kilobase-pair subfragment of the genome.
    Proc Natl Acad Sci U S A. 1984 Jan;81(1):43-7 PMID: 6320171
  28. Sequence of the murine and human cellular myc oncogenes and two modes of myc transcription resulting from chromosome translocation in B lymphoid tumours.
    EMBO J. 1983;2(12):2375-83 PMID: 6321164
  29. Activation of the c-myc gene by translocation: a model for translational control.
    Proc Natl Acad Sci U S A. 1983 Dec;80(24):7476-80 PMID: 6324175
  30. Immortalization of monkey epithelial cells by specific fragments of Epstein-Barr virus DNA.
    Nature. 1984 May 3-9;309(5963):78-82 PMID: 6325929
  31. Nucleotide sequence of an mRNA transcribed in latent growth-transforming virus infection indicates that it may encode a membrane protein.
    J Virol. 1984 Aug;51(2):411-9 PMID: 6086953
  32. DNA sequence and expression of the B95-8 Epstein-Barr virus genome.
    Nature. 1984 Jul 19-25;310(5974):207-11 PMID: 6087149
  33. Identification of Epstein-Barr virus sequences that encode a nuclear antigen expressed in latently infected lymphocytes.
    Proc Natl Acad Sci U S A. 1984 Jul;81(14):4373-7 PMID: 6087322
  34. Antibodies against a synthetic peptide identify the Epstein-Barr virus-determined nuclear antigen.
    Proc Natl Acad Sci U S A. 1984 Aug;81(15):4652-6 PMID: 6205400
  35. Spliced RNA from the IR1-U2 region of Epstein-Barr virus: presence of an open reading frame for a repetitive polypeptide.
    EMBO J. 1984 Aug;3(8):1913-7 PMID: 6090131
  36. Stable replication of plasmids derived from Epstein-Barr virus in various mammalian cells.
    Nature. 1985 Feb 28-Mar 6;313(6005):812-5 PMID: 2983224
  37. Differential efficiencies of in vitro translation of mouse c-myc transcripts differing in the 5' untranslated region.
    Proc Natl Acad Sci U S A. 1985 Apr;82(8):2315-9 PMID: 3857582
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.17.7103
Published
1986-09-11
Pages
7103-14
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311721
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/14.17.7103 PMC Full Text View journal details
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