Home LiteratureArticle Details
PMID: 3025831 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

An Epstein-Barr virus transcript from a latently infected, growth-transformed B-cell line encodes a highly repetitive polypeptide.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 24 ·1986-12-00 ·Pages 9298-302

Speck SH, Pfitzner A, Strominger JL

Abstract

By screening a cDNA library prepared from poly(A)+ RNA isolated from an Epstein-Barr virus latently infected, growth-transformed human B-lymphoblastoid cell line, we have recovered a clone corresponding to a highly spliced viral transcript encoded largely by the major internal repeat (IR1). The 5' region contains one copy of a 26-base-pair (bp) exon (W0) [which is 28 bp downstream from a CAATT-(N)34TATAAA sequence (N, unspecified base)] and seven copies of two small exons (W1, 66 bp; W2, 132 bp). In addition, there are three exons from the "unique" region of the BamHI Y fragment of the viral genome. Two other cDNA clones that have been described, corresponding to latent viral transcripts, share homology in their 5' regions with this clone and are clearly divergent at their 3' ends. The cDNA clone described in this paper contains one long open reading frame that extends through the repeat element. In vitro transcription and translation of this open reading frame yielded a 62-kDa polypeptide that could be immunoprecipitated by an Epstein-Barr virus-positive human serum.

MeSH Terms
B-Lymphocytes Base Sequence Cell Transformation, Viral Cloning, Molecular DNA/genetics DNA, Viral/genetics Genes, Viral Herpesvirus 4, Human/genetics Protein Biosynthesis RNA, Viral/genetics Repetitive Sequences, Nucleic Acid Transcription, Genetic
Chemicals
DNA, Viral RNA, Viral DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Speck S H
Pfitzner A
Strominger J L
References (36)
36 references, click to expand
  1. A second nuclear protein is encoded by Epstein-Barr virus in latent infection.
    Science. 1985 Mar 8;227(4691):1238-40 PMID: 2983420
  2. Stable replication of plasmids derived from Epstein-Barr virus in various mammalian cells.
    Nature. 1985 Feb 28-Mar 6;313(6005):812-5 PMID: 2983224
  3. A third viral nuclear protein in lymphoblasts immortalized by Epstein-Barr virus.
    Proc Natl Acad Sci U S A. 1985 Sep;82(17):5944-8 PMID: 2994055
  4. Identification of a nuclear localization signal of a yeast ribosomal protein.
    Proc Natl Acad Sci U S A. 1985 Oct;82(19):6561-5 PMID: 3931077
  5. GCN4 protein, synthesized in vitro, binds HIS3 regulatory sequences: implications for general control of amino acid biosynthetic genes in yeast.
    Cell. 1985 Nov;43(1):177-88 PMID: 3907851
  6. Analysis of the transcript encoding the latent Epstein-Barr virus nuclear antigen I: a potentially polycistronic message generated by long-range splicing of several exons.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8305-9 PMID: 3001694
  7. Arg-Gly-Asp: a versatile cell recognition signal.
    Cell. 1986 Feb 28;44(4):517-8 PMID: 2418980
  8. Four virally determined nuclear antigens are expressed in Epstein-Barr virus-transformed cells.
    Proc Natl Acad Sci U S A. 1986 Mar;83(5):1499-503 PMID: 3485288
  9. An Epstein-Barr virus transcription unit is at least 84 kilobases long.
    Nucleic Acids Res. 1986 Mar 25;14(6):2611-20 PMID: 3008094
  10. Nucleotide sequences of mRNAs encoding Epstein-Barr virus nuclear proteins: a probable transcriptional initiation site.
    Proc Natl Acad Sci U S A. 1986 Jul;83(14):5096-100 PMID: 3460083
  11. An Epstein-Barr virus (EBV)-determined nuclear antigen (EBNA5) partly encoded by the transformation-associated Bam WYH region of EBV DNA: preferential expression in lymphoblastoid cell lines.
    Proc Natl Acad Sci U S A. 1986 Sep;83(17):6641-5 PMID: 3018741
  12. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  13. Maturation of the head of bacteriophage T4. I. DNA packaging events.
    J Mol Biol. 1973 Nov 15;80(4):575-99 PMID: 4204102
  14. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  15. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.
    J Immunol. 1975 Dec;115(6):1617-24 PMID: 1102604
  16. Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4370-4 PMID: 270678
  17. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  18. Tumor initiators and promoters in the induction of Epstein-Barr virus.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):782-5 PMID: 218219
  19. Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping.
    Methods Enzymol. 1980;65(1):718-49 PMID: 6154876
  20. Epstein-Barr virus RNA. V. Viral RNA in a restringently infected, growth-transformed cell line.
    J Virol. 1980 Nov;36(2):506-18 PMID: 6253674
  21. Cloning and mapping of BamHi endonuclease fragments of DNA from the transforming B95-8 strain of Epstein-Barr virus.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3860-4 PMID: 6253994
  22. Epstein-Barr virus RNA VII: size and direction of transcription of virus-specified cytoplasmic RNAs in a transformed cell line.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1930-4 PMID: 6112750
  23. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  24. A catalogue of splice junction sequences.
    Nucleic Acids Res. 1982 Jan 22;10(2):459-72 PMID: 7063411
  25. Intracellular transport of SV40 large tumor antigen: a mutation which abolishes migration to the nucleus does not prevent association with the cell surface.
    Virology. 1982 May;119(1):169-84 PMID: 6280381
  26. Stable expression in mouse cells of nuclear neoantigen after transfer of a 3.4-megadalton cloned fragment of Epstein-Barr virus DNA.
    Proc Natl Acad Sci U S A. 1982 Sep;79(18):5688-92 PMID: 6291059
  27. An unusually high-titer human anti-Epstein Barr virus (EBV) serum and its use in the study of EBV-specific proteins synthesized in vitro and in vivo.
    J Immunol. 1983 Feb;130(2):919-24 PMID: 6294181
  28. Simple repeat array in Epstein-Barr virus DNA encodes part of the Epstein-Barr nuclear antigen.
    Science. 1983 Jun 24;220(4604):1396-8 PMID: 6304878
  29. An integrated and simplified approach to cloning into plasmids and single-stranded phages.
    Methods Enzymol. 1983;101:78-89 PMID: 6310343
  30. A simple and very efficient method for generating cDNA libraries.
    Gene. 1983 Nov;25(2-3):263-9 PMID: 6198242
  31. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
    Nucleic Acids Res. 1984 Jan 25;12(2):857-72 PMID: 6694911
  32. Nucleotide sequence of an mRNA transcribed in latent growth-transforming virus infection indicates that it may encode a membrane protein.
    J Virol. 1984 Aug;51(2):411-9 PMID: 6086953
  33. Spliced RNA from the IR1-U2 region of Epstein-Barr virus: presence of an open reading frame for a repetitive polypeptide.
    EMBO J. 1984 Aug;3(8):1913-7 PMID: 6090131
  34. Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
    Nucleic Acids Res. 1984 Sep 25;12(18):7057-70 PMID: 6207484
  35. A short amino acid sequence able to specify nuclear location.
    Cell. 1984 Dec;39(3 Pt 2):499-509 PMID: 6096007
  36. An improved DNA sequencing strategy.
    Anal Biochem. 1985 May 15;147(1):114-9 PMID: 2992313
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.24.9298
Published
1986-12-00
Pages
9298-302
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC387125
Subset
IM
Grants
NCI NIH HHS · 5F32CA07174 · United States
NCI NIH HHS · 5PO1CA21082 · United States
Databases
GENBANK
M14976
External Links
PubMed source DOI: 10.1073/pnas.83.24.9298 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2