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PMID: 2431275 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Expression and localization of Drosophila melanogaster hsp70 cognate proteins.

Molecular and cellular biology ·Vol. 6 ·No. 4 ·1986-04-00 ·Pages 1187-203

Palter KB, Watanabe M, Stinson L, Mahowald AP, Craig EA

Abstract

Monoclonal antibodies have been used to identify three proteins in Drosophila melanogaster that share antigenic determinants with the major heat shock proteins hsp70 and hsp68. While two of the proteins are major proteins at all developmental stages, one heat shock cognate protein, hsc70, is especially enriched in embryos. hsc70 is shown to be the product of a previously identified gene, Hsc4. We have examined the levels of hsp70-related proteins in adult flies and larvae during heat shock and recovery. At maximal induction in vivo, hsp70 and hsp68 never reach the basal levels of the major heat shock cognate proteins. Monoclonal antibodies to hsc70 have been used to localize it to a meshwork of cytoplasmic fibers that are heavily concentrated around the nucleus.

MeSH Terms
Animals Antibodies, Monoclonal Cells, Cultured DNA/metabolism Drosophila melanogaster/genetics Epitopes/analysis Genes Heat-Shock Proteins/analysis,genetics Larva Transcription, Genetic
Chemicals
Antibodies, Monoclonal Epitopes Heat-Shock Proteins DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Palter K B
Watanabe M
Stinson L
Mahowald A P
Craig E A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.4.1187-1203.1986
Published
1986-04-00
Pages
1187-203
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367631
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.6.4.1187-1203.1986 PMC Full Text View journal details
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