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PMID: 2422398 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Passive protection against rotavirus-induced diarrhea by monoclonal antibodies to surface proteins vp3 and vp7.

Journal of virology ·Vol. 58 ·No. 2 ·1986-05-00 ·Pages 700-3

Offit PA, Shaw RD, Greenberg HB

Abstract

Monoclonal antibodies directed against two rotavirus surface proteins (vp3 and vp7) as well as a rotavirus inner capsid protein (vp6) were tested for their ability to protect suckling mice against virulent rotavirus challenge. Monoclonal antibodies to two distinct epitopes of vp7 of simian rotavirus strain RRV neutralized RRV in vitro and passively protected suckling mice against RRV challenge. A monoclonal antibody directed against vp3 of porcine rotavirus strain OSU neutralized three distinct serotypes in vitro (OSU, RRV, and UK) and passively protected suckling mice against OSU, RRV, and UK virus-induced diarrhea. The role of vp3 in eliciting protection against heterotypic rotavirus challenge should be considered when developing a vaccine with cloned rotavirus genes. Alternatively, immunization with a reassortant rotavirus containing vp3 and vp7 from two antigenically distinct rotavirus parents might protect against diarrhea induced by two or more rotavirus serotypes.

MeSH Terms
Animals Animals, Suckling Antibodies, Monoclonal Antigens, Viral/immunology Capsid/immunology Diarrhea/etiology,prevention & control Epitopes Immunization, Passive Membrane Proteins/immunology Mice Rotavirus/classification,immunology Rotavirus Infections/prevention & control Serotyping Viral Proteins/immunology
Chemicals
Antibodies, Monoclonal Antigens, Viral Epitopes Membrane Proteins Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Offit P A
Shaw R D
Greenberg H B
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36 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.58.2.700-703.1986
Published
1986-05-00
Pages
700-3
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC252967
Subset
IM
Grants
NIAID NIH HHS · 1 R23 AI-21065-01 · United States
NIAID NIH HHS · R22 AI-21362-01 · United States
External Links
PubMed source DOI: 10.1128/JVI.58.2.700-703.1986 PMC Full Text View journal details
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