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PMID: 6312065 Published · ppublish English Journal Article

Production and preliminary characterization of monoclonal antibodies directed at two surface proteins of rhesus rotavirus.

Journal of virology ·Vol. 47 ·No. 2 ·1983-08-00 ·Pages 267-75

Greenberg HB, Valdesuso J, van Wyke K, Midthun K, Walsh M, McAuliffe V, Wyatt RG, Kalica AR, Flores J, Hoshino Y

Abstract

A series of monoclonal antibodies was isolated which reacted with one of two major surface proteins of rhesus rotavirus. Thirty-six monoclonal antibodies immunoprecipitated the 82-kilodalton outer capsid protein, the product of the fourth gene, the viral hemagglutinin. These monoclonal antibodies exhibited hemagglutination inhibition activity and neutralized rhesus rotavirus to moderate or high titer. Three monoclonal antibodies immunoprecipitated the 38-kilodalton outer capsid glycoprotein, the eighth or ninth gene product. These three monoclonal antibodies neutralized rhesus rotavirus to high titer and also inhibited viral hemagglutination.

MeSH Terms
Animals Antibodies, Monoclonal/analysis Antibodies, Viral/analysis Capsid/immunology Cattle Glycoproteins/immunology Hemagglutination Inhibition Tests Hemagglutination Tests Hemagglutinins, Viral/immunology Macaca mulatta Neutralization Tests Radioimmunoassay Rotavirus/immunology Viral Proteins/immunology
Chemicals
Antibodies, Monoclonal Antibodies, Viral Glycoproteins Hemagglutinins, Viral Viral Proteins
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Greenberg H B
Valdesuso J
van Wyke K
Midthun K
Walsh M
McAuliffe V
Wyatt R G
Kalica A R
Flores J
Hoshino Y
References (30)
30 references, click to expand
  1. Recombination of influenza A viruses of human and animal origin.
    Science. 1968 Apr 5;160(3823):74-6 PMID: 5642310
  2. Microtiter solid-phase radioimmunoassay for hepatitis B antigen.
    Appl Microbiol. 1973 Oct;26(4):478-84 PMID: 4201649
  3. Neonatal calf diarrhoea: identification of a reovirus-like (rotavirus) agent in faeces by immunofluorescence and immune electron microscopy.
    Br Vet J. 1975 Sep-Oct;131(5):528-35 PMID: 172192
  4. Ribonucleic acid polymerase activity associated with purified calf rotavirus.
    J Gen Virol. 1977 Sep;36(3):395-402 PMID: 21225
  5. Pathogenesis of porcine rotaviral infection in experimentally inoculated gnotobiotic pigs.
    Am J Vet Res. 1978 Feb;39(2):213-20 PMID: 204232
  6. Hemagglutination by simian rotavirus.
    J Clin Microbiol. 1978 Mar;7(3):314-5 PMID: 206575
  7. The rotaviruses.
    Arch Virol. 1978;57(1):1-23 PMID: 77663
  8. Enzyme-linked immunosorbent assay for identification of rotaviruses from different animal species.
    Science. 1978 Jul 21;201(4352):259-62 PMID: 208150
  9. Antigenic comparisons of two new rotaviruses from rhesus monkeys.
    J Clin Microbiol. 1980 Feb;11(2):202-3 PMID: 6244332
  10. In vitro transcription and translation of simian rotavirus SA11 gene products.
    J Virol. 1980 Mar;33(3):1111-21 PMID: 6245262
  11. Coding assignments of double-stranded RNA segments of SA 11 rotavirus established by in vitro translation.
    J Virol. 1980 Mar;33(3):976-82 PMID: 6245278
  12. Structural polypeptides of simian rotavirus SA11 and the effect of trypsin.
    J Virol. 1981 Jan;37(1):156-60 PMID: 6260970
  13. Rescue of noncultivatable human rotavirus by gene reassortment during mixed infection with ts mutants of a cultivatable bovine rotavirus.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):420-4 PMID: 6264442
  14. Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigens.
    Virology. 1981 Jul 30;112(2):385-90 PMID: 6266134
  15. Characterization of anti-reovirus immunoglobulins secreted by cloned hybridoma cell lines.
    Virology. 1981 Jan 15;108(1):134-46 PMID: 7269233
  16. The interaction of a series of hybridoma IgGs with reovirus particles. Demonstration that the core protein lambda 2 is exposed on the particle surface.
    Virology. 1981 Jan 15;108(1):147-55 PMID: 7269234
  17. Proteolytic enhancement of rotavirus infectivity: molecular mechanisms.
    J Virol. 1981 Sep;39(3):879-88 PMID: 6270356
  18. Preparation and characterization of antisera to electrophoretically purified SA11 virus polypeptides.
    Infect Immun. 1981 Dec;34(3):641-7 PMID: 6174448
  19. Evidence for functional domains on the reovirus type 3 hemagglutinin.
    Virology. 1982 Feb;117(1):146-55 PMID: 6175082
  20. The molecular biology of rotaviruses. II. Identification of the protein-coding assignments of calf rotavirus genome RNA species.
    Virology. 1982 Mar;117(2):435-43 PMID: 6278745
  21. Attenuated reovirus type 3 strains generated by selection of haemagglutinin antigenic variants.
    Nature. 1982 May 6;297(5861):68-70 PMID: 6175910
  22. Isolation and characterization of a canine rotavirus.
    Arch Virol. 1982;72(1-2):113-25 PMID: 6179502
  23. Rescue and serotypic characterization of noncultivable human rotavirus by gene reassortment.
    Infect Immun. 1982 Jul;37(1):104-9 PMID: 6286486
  24. Definition of human rotavirus serotypes by plaque reduction assay.
    Infect Immun. 1982 Jul;37(1):110-5 PMID: 6286487
  25. Use of transcription probes for genotyping rotavirus reassortants.
    Virology. 1982 Sep;121(2):288-95 PMID: 6289518
  26. Identification of a neutralization-specific antigen of a calf rotavirus.
    J Gen Virol. 1982 Oct;62 (Pt 2):297-311 PMID: 6292353
  27. Purification of an outer capsid glycoprotein of neonatal calf diarrhea virus and preparation of its antisera.
    Infect Immun. 1983 Jan;39(1):155-8 PMID: 6295955
  28. Serological analysis of the subgroup protein of rotavirus, using monoclonal antibodies.
    Infect Immun. 1983 Jan;39(1):91-9 PMID: 6185436
  29. Identification of the rotaviral gene that codes for hemagglutination and protease-enhanced plaque formation.
    Virology. 1983 Feb;125(1):194-205 PMID: 6299006
  30. Gene coding assignments for growth restriction, neutralization and subgroup specificities of the W and DS-1 strains of human rotavirus.
    J Gen Virol. 1983 Feb;64 (Pt 2):313-20 PMID: 6300286
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.47.2.267-275.1983
Published
1983-08-00
Pages
267-75
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255258
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.47.2.267-275.1983 PMC Full Text View journal details
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