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PMID: 24183695 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

Fluorescence spectroscopy of rhodopsins: insights and approaches.

Biochimica et biophysica acta ·Vol. 1837 ·No. 5 ·2014-05-00 ·Pages 694-709

Alexiev U, Farrens DL

Abstract

Fluorescence spectroscopy has become an established tool at the interface of biology, chemistry and physics because of its exquisite sensitivity and recent technical advancements. However, rhodopsin proteins present the fluorescence spectroscopist with a unique set of challenges and opportunities due to the presence of the light-sensitive retinal chromophore. This review briefly summarizes some approaches that have successfully met these challenges and the novel insights they have yielded about rhodopsin structure and function. We start with a brief overview of fluorescence fundamentals and experimental methodologies, followed by more specific discussions of technical challenges rhodopsin proteins present to fluorescence studies. Finally, we end by discussing some of the unique insights that have been gained specifically about visual rhodopsin and its interactions with affiliate proteins through the use of fluorescence spectroscopy. This article is part of a Special Issue entitled: Retinal Proteins - You can teach an old dog new tricks.

Keywords
Fluorescence spectroscopy Retinal protein Site-directed fluorescence labeling Visual rhodopsin
MeSH Terms
Amino Acid Sequence Animals Cattle Fluorescence Polarization Fluorescent Dyes/chemistry Humans Models, Molecular Molecular Sequence Data Photons Protein Structure, Secondary Protein Structure, Tertiary Retinaldehyde/chemistry,metabolism Rhodopsin/chemistry,metabolism Spectrometry, Fluorescence/methods,statistics & numerical data
Chemicals
Fluorescent Dyes Rhodopsin Retinaldehyde
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Alexiev Ulrike
Physics Department, Freie Universität Berlin, Berlin, Germany. Electronic address: alexiev@physik.fu-berlin.de.
Farrens David L
Departments of Biochemistry and Molecular Biology, Oregon Health Sciences University, USA.
References (140)
140 references, click to expand
  1. The magnitude of the light-induced conformational change in different rhodopsins correlates with their ability to activate G proteins.
    J Biol Chem. 2009 Jul 31;284(31):20676-83 PMID: 19497849
  2. Light-activated rhodopsin induces structural binding motif in G protein alpha subunit.
    Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4270-5 PMID: 9539726
  3. Transducin-alpha C-terminal peptide binding site consists of C-D and E-F loops of rhodopsin.
    J Biol Chem. 1997 Mar 7;272(10):6519-24 PMID: 9045677
  4. Conformational dynamics of helix 8 in the GPCR rhodopsin controls arrestin activation in the desensitization process.
    Proc Natl Acad Sci U S A. 2011 Nov 15;108(46):18690-5 PMID: 22039220
  5. Activation and molecular recognition of the GPCR rhodopsin--insights from time-resolved fluorescence depolarisation and single molecule experiments.
    Eur J Cell Biol. 2012 Apr;91(4):300-10 PMID: 21803442
  6. Surface charge of bacteriorhodopsin detected with covalently bound pH indicators at selected extracellular and cytoplasmic sites.
    Biochemistry. 1994 Jan 11;33(1):298-306 PMID: 8286351
  7. Ultrasensitive measurements of microbial rhodopsin photocycles using photochromic FRET.
    Photochem Photobiol. 2012 Jan-Feb;88(1):90-7 PMID: 22010969
  8. Ligand channeling within a G-protein-coupled receptor. The entry and exit of retinals in native opsin.
    J Biol Chem. 2003 Jul 4;278(27):24896-24903 PMID: 12707280
  9. Time-resolved detection of transient movement of helices F and G in doubly spin-labeled bacteriorhodopsin.
    Biophys J. 2001 Jun;80(6):2856-66 PMID: 11371459
  10. Rhodopsin activation exposes a key hydrophobic binding site for the transducin alpha-subunit C terminus.
    J Biol Chem. 2004 Jul 9;279(28):29767-73 PMID: 15070895
  11. Rhodopsin self-associates in asolectin liposomes.
    Proc Natl Acad Sci U S A. 2006 Feb 28;103(9):3060-5 PMID: 16492772
  12. Mechanism of voltage-sensitive fluorescence in a microbial rhodopsin.
    Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):5939-44 PMID: 23530193
  13. High-throughput protein structural analysis using site-directed fluorescence labeling and the bimane derivative (2-pyridyl)dithiobimane.
    Biochemistry. 2004 Jul 27;43(29):9426-38 PMID: 15260485
  14. Monitoring the conformational changes of photoactivated rhodopsin from microseconds to seconds by transient fluorescence spectroscopy.
    Biochemistry. 2008 Nov 4;47(44):11518-27 PMID: 18847221
  15. Sensory rhodopsin II: functional insights from structure.
    Curr Opin Struct Biol. 2002 Aug;12(4):540-6 PMID: 12163079
  16. Site of G protein binding to rhodopsin mapped with synthetic peptides from the alpha subunit.
    Science. 1988 Aug 12;241(4867):832-5 PMID: 3136547
  17. Conformational changes in rhodopsin. Movement of helix f detected by site-specific chemical labeling and fluorescence spectroscopy.
    J Biol Chem. 1999 Jan 15;274(3):1683-90 PMID: 9880548
  18. Intramolecular energy transfer in rhodopsin.
    Vision Res. 1967 Nov;7(11):811-8 PMID: 5615775
  19. Mislocalization and degradation of human P23H-rhodopsin-GFP in a knockin mouse model of retinitis pigmentosa.
    Invest Ophthalmol Vis Sci. 2011 Dec 28;52(13):9728-36 PMID: 22110080
  20. A key agonist-induced conformational change in the cannabinoid receptor CB1 is blocked by the allosteric ligand Org 27569.
    J Biol Chem. 2012 Sep 28;287(40):33873-82 PMID: 22846992
  21. Intermediates in the folding of the membrane protein bacteriorhodopsin.
    Nat Struct Biol. 1995 Feb;2(2):139-43 PMID: 7749918
  22. Arrestin can act as a regulator of rhodopsin photochemistry.
    Vision Res. 2006 Dec;46(27):4532-46 PMID: 17069872
  23. Site-specific in vitro and in vivo incorporation of molecular probes to study G-protein-coupled receptors.
    Curr Opin Chem Biol. 2011 Jun;15(3):392-8 PMID: 21571577
  24. Proximity relationships in rhodopsin.
    Proc Natl Acad Sci U S A. 1972 May;69(5):1104-8 PMID: 4504322
  25. Rapid release of retinal from a cone visual pigment following photoactivation.
    Biochemistry. 2012 May 22;51(20):4117-25 PMID: 22217337
  26. Responses of retinal rods to single photons.
    J Physiol. 1979 Mar;288:613-34 PMID: 112243
  27. Structure and function in rhodopsin. Measurement of the rate of metarhodopsin II decay by fluorescence spectroscopy.
    J Biol Chem. 1995 Mar 10;270(10):5073-6 PMID: 7890614
  28. Homogeneous time-resolved fluorescence assay to probe folded G protein-coupled receptors.
    Methods Enzymol. 2013;522:169-89 PMID: 23374186
  29. Energy transfer in rhodopsin, N-retinyl-opsin, and rod outer segments.
    Proc Natl Acad Sci U S A. 1971 Apr;68(4):713-6 PMID: 5279513
  30. G protein-coupled receptor activation: analysis of a highly constrained, "straitjacketed" rhodopsin.
    Biochemistry. 2000 Jul 11;39(27):7938-42 PMID: 10891074
  31. GPCR monomers and oligomers: it takes all kinds.
    Trends Neurosci. 2008 Feb;31(2):74-81 PMID: 18199492
  32. The multitalented microbial sensory rhodopsins.
    Trends Microbiol. 2006 Nov;14(11):480-7 PMID: 17005405
  33. Escaping the flatlands: new approaches for studying the dynamic assembly and activation of GPCR signaling complexes.
    Trends Pharmacol Sci. 2011 Jul;32(7):410-9 PMID: 21497404
  34. Picosecond multidimensional fluorescence spectroscopy: a tool to measure real-time protein dynamics during function.
    Photochem Photobiol. 2007 Mar-Apr;83(2):378-84 PMID: 17117889
  35. Förster resonance energy transfer as a tool to study photoreceptor biology.
    J Biomed Opt. 2010 Nov-Dec;15(6):067001 PMID: 21198205
  36. A photosensitive product of sodium borohydride reduction of bacteriorhodopsin.
    FEBS Lett. 1976 Jan 15;61(2):128-34 PMID: 1248615
  37. NMR spectroscopy in studies of light-induced structural changes in mammalian rhodopsin: applicability of solution (19)F NMR.
    Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13744-9 PMID: 10570143
  38. Distinct loops in arrestin differentially regulate ligand binding within the GPCR opsin.
    Nat Commun. 2012;3:995 PMID: 22871814
  39. Optical recording of action potentials in mammalian neurons using a microbial rhodopsin.
    Nat Methods. 2011 Nov 27;9(1):90-5 PMID: 22120467
  40. Mechanism of isomerization of rhodopsin studied by use of 11-cis-locked rhodopsin analogues excited with a picosecond laser pulse.
    Biochemistry. 1989 Jul 25;28(15):6460-7 PMID: 2790007
  41. Microbial rhodopsins: scaffolds for ion pumps, channels, and sensors.
    Results Probl Cell Differ. 2008;45:73-122 PMID: 17898961
  42. Pre-lumirhodopsin and the bleaching of visual pigments.
    Nature. 1963 Mar 30;197:1279-86 PMID: 14002749
  43. Rhodopsin activation blocked by metal-ion-binding sites linking transmembrane helices C and F.
    Nature. 1996 Sep 26;383(6598):347-50 PMID: 8848049
  44. Simulation of fluorescence anisotropy experiments: probing protein dynamics.
    Biophys J. 2005 Dec;89(6):3757-70 PMID: 16169987
  45. Rhodopsin-G-protein interactions monitored by resonance energy transfer.
    Biochemistry. 1989 Feb 21;28(4):1711-8 PMID: 2497769
  46. REACTION OF THE RHODOPSIN CHROMOPHORE WITH SODIUM BOROHYDRIDE.
    Nature. 1965 Jan 16;205:254-7 PMID: 14270706
  47. Site-specific incorporation of keto amino acids into functional G protein-coupled receptors using unnatural amino acid mutagenesis.
    J Biol Chem. 2008 Jan 18;283(3):1525-1533 PMID: 17993461
  48. Multiple steps of phosphorylation of activated rhodopsin can account for the reproducibility of vertebrate rod single-photon responses.
    J Gen Physiol. 2003 Oct;122(4):419-44 PMID: 12975449
  49. First steps of retinal photoisomerization in proteorhodopsin.
    Biophys J. 2006 Jul 1;91(1):255-62 PMID: 16603495
  50. Effect of channel mutations on the uptake and release of the retinal ligand in opsin.
    Proc Natl Acad Sci U S A. 2012 Apr 3;109(14):5247-52 PMID: 22431612
  51. Time-resolved detection of transient movement of helix F in spin-labelled pharaonis sensory rhodopsin II.
    J Mol Biol. 2000 Aug 25;301(4):881-91 PMID: 10966793
  52. Effect of ethanol and osmotic stress on receptor conformation. Reduced water activity amplifies the effect of ethanol on metarhodopsin II formation.
    J Biol Chem. 2000 Feb 25;275(8):5355-60 PMID: 10681509
  53. Rapid long-range proton diffusion along the surface of the purple membrane and delayed proton transfer into the bulk.
    Proc Natl Acad Sci U S A. 1995 Jan 17;92(2):372-6 PMID: 7831293
  54. Efficient coupling of transducin to monomeric rhodopsin in a phospholipid bilayer.
    J Biol Chem. 2008 Feb 15;283(7):4387-94 PMID: 18033822
  55. Crystal structure of rhodopsin: A G protein-coupled receptor.
    Science. 2000 Aug 4;289(5480):739-45 PMID: 10926528
  56. A three-dimensional difference map of the N intermediate in the bacteriorhodopsin photocycle: part of the F helix tilts in the M to N transition.
    Biochemistry. 1996 May 7;35(18):5870-8 PMID: 8639548
  57. Molecular basis of transmembrane signalling by sensory rhodopsin II-transducer complex.
    Nature. 2002 Oct 3;419(6906):484-7 PMID: 12368857
  58. Algal sensory photoreceptors.
    Annu Rev Plant Biol. 2008;59:167-89 PMID: 18444900
  59. The cytoplasmic membrane-proximal domain of the HtrII transducer interacts with the E-F loop of photoactivated Natronomonas pharaonis sensory rhodopsin II.
    J Biol Chem. 2004 Oct 8;279(41):42970-6 PMID: 15262967
  60. Lipid protein interactions couple protonation to conformation in a conserved cytosolic domain of G protein-coupled receptors.
    J Biol Chem. 2009 Oct 16;284(42):28801-9 PMID: 19706606
  61. Constraints on the flexibility of bacteriorhodopsin's carboxyl-terminal tail at the purple membrane surface.
    Biochemistry. 1983 Jan 4;22(1):5-12 PMID: 6830763
  62. The surface of visual arrestin that binds to rhodopsin.
    Mol Vis. 2004 Jun 15;10:392-8 PMID: 15215746
  63. A constitutively activating mutation alters the dynamics and energetics of a key conformational change in a ligand-free G protein-coupled receptor.
    J Biol Chem. 2013 Sep 27;288(39):28207-16 PMID: 23940032
  64. Rapid and reproducible deactivation of rhodopsin requires multiple phosphorylation sites.
    Neuron. 2000 Oct;28(1):153-64 PMID: 11086991
  65. Fluorescence quantum yield of visual pigments: evidence for subpicosecond isomerization rates.
    Proc Natl Acad Sci U S A. 1984 Aug;81(15):4790-4 PMID: 6589626
  66. Characterization of membrane protein non-native states. 1. Extent of unfolding and aggregation of rhodopsin in the presence of chemical denaturants.
    Biochemistry. 2010 Aug 3;49(30):6317-28 PMID: 20575534
  67. Phosphorylation modulates the affinity of light-activated rhodopsin for G protein and arrestin.
    Biochemistry. 2000 May 16;39(19):5738-49 PMID: 10801324
  68. The molecular basis of visual excitation.
    Nature. 1968 Aug 24;219(5156):800-7 PMID: 4876934
  69. Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy.
    J Mol Biol. 1990 Jun 20;213(4):899-929 PMID: 2359127
  70. The archaeal sensory rhodopsin II/transducer complex: a model for transmembrane signal transfer.
    FEBS Lett. 2004 Apr 30;564(3):219-24 PMID: 15111099
  71. Sensory rhodopsin II/transducer complex formation in detergent and in lipid bilayers studied with FRET.
    Biochim Biophys Acta. 2009 Feb;1788(2):522-31 PMID: 19094962
  72. Fluorescence quenching by photoinduced electron transfer: a reporter for conformational dynamics of macromolecules.
    Chemphyschem. 2009 Jul 13;10(9-10):1389-98 PMID: 19475638
  73. Fluorescence quenching studies with proteins.
    Anal Biochem. 1981 Jul 1;114(2):199-227 PMID: 7030122
  74. Dynamics of light-induced conformational changes of the phoborhodopsin/transducer complex formed in the n-dodecyl beta-D-maltoside micelle.
    Biochemistry. 2007 May 8;46(18):5349-57 PMID: 17432830
  75. Specific expression of channelrhodopsin-2 in single neurons of Caenorhabditis elegans.
    PLoS One. 2012;7(8):e43164 PMID: 22952643
  76. Two rhodopsins mediate phototaxis to low- and high-intensity light in Chlamydomonas reinhardtii.
    Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8689-94 PMID: 12060707
  77. Origin of Fluorescence in 11-cis Locked Bovine Rhodopsin.
    J Chem Theory Comput. 2012 Aug 14;8(8):2559-63 PMID: 26592102
  78. pH-dependent photoisomerization of retinal in proteorhodopsin.
    Biochemistry. 2005 Feb 15;44(6):1800-6 PMID: 15697205
  79. A theory of fluorescence polarization decay in membranes.
    Biophys J. 1977 Dec;20(3):289-305 PMID: 922121
  80. Oligomerization of G protein-coupled receptors: past, present, and future.
    Biochemistry. 2004 Dec 21;43(50):15643-56 PMID: 15595821
  81. Organization of rhodopsin molecules in native membranes of rod cells--an old theoretical model compared to new experimental data.
    J Mol Model. 2005 Sep;11(4-5):385-91 PMID: 15928919
  82. Rhodopsin in nanodiscs has native membrane-like photointermediates.
    Biochemistry. 2011 Jun 7;50(22):5086-91 PMID: 21539361
  83. Photoreceptor signaling: supporting vision across a wide range of light intensities.
    J Biol Chem. 2012 Jan 13;287(3):1620-6 PMID: 22074925
  84. Requirement of rigid-body motion of transmembrane helices for light activation of rhodopsin.
    Science. 1996 Nov 1;274(5288):768-70 PMID: 8864113
  85. Evidence for structural changes in carboxyl-terminal peptides of transducin alpha-subunit upon binding a soluble mimic of light-activated rhodopsin.
    Biochemistry. 2003 Jan 21;42(2):302-11 PMID: 12525157
  86. Fluorescence spectra of the intermediates of rhodopsin bleaching.
    Photochem Photobiol. 1969 Jun;9(6):565-70 PMID: 5807949
  87. Functional interaction structures of the photochromic retinal protein rhodopsin.
    Photochem Photobiol Sci. 2010 Feb;9(2):226-33 PMID: 20126799
  88. Electron diffraction analysis of structural changes in the photocycle of bacteriorhodopsin.
    EMBO J. 1993 Jan;12(1):1-8 PMID: 8428572
  89. Translational diffusion and interaction of a photoreceptor and its cognate transducer observed in giant unilamellar vesicles by using dual-focus FCS.
    Chembiochem. 2009 Jul 20;10(11):1823-9 PMID: 19551796
  90. Tryptophan fluorescence quenching as a monitor for the protein conformation changes occurring during the photocycle of bacteriorhodopsin under different perturbations.
    Proc Natl Acad Sci U S A. 1989 Aug;86(15):5815-9 PMID: 2762298
  91. Signaling states of rhodopsin. Formation of the storage form, metarhodopsin III, from active metarhodopsin II.
    J Biol Chem. 2003 Jan 31;278(5):3162-9 PMID: 12427735
  92. Dynamics of arrestin-rhodopsin interactions: arrestin and retinal release are directly linked events.
    J Biol Chem. 2005 Feb 25;280(8):6861-71 PMID: 15591052
  93. The contribution of a covalently bound cofactor to the folding and thermodynamic stability of an integral membrane protein.
    J Mol Biol. 2010 Nov 5;403(4):630-42 PMID: 20850459
  94. An archaeal photosignal-transducing module mediates phototaxis in Escherichia coli.
    J Bacteriol. 2001 Nov;183(21):6365-71 PMID: 11591681
  95. Rapid incorporation of functional rhodopsin into nanoscale apolipoprotein bound bilayer (NABB) particles.
    J Mol Biol. 2008 Apr 4;377(4):1067-81 PMID: 18313692
  96. Linkage between the intramembrane H-bond network around aspartic acid 83 and the cytosolic environment of helix 8 in photoactivated rhodopsin.
    J Mol Biol. 2007 Mar 2;366(4):1129-41 PMID: 17196983
  97. Structure and function in rhodopsin: effects of disulfide cross-links in the cytoplasmic face of rhodopsin on transducin activation and phosphorylation by rhodopsin kinase.
    Biochemistry. 1999 Sep 28;38(39):12893-8 PMID: 10504260
  98. Crystal structure of sensory rhodopsin II at 2.4 angstroms: insights into color tuning and transducer interaction.
    Science. 2001 Aug 24;293(5534):1499-503 PMID: 11452084
  99. Monomeric rhodopsin is the minimal functional unit required for arrestin binding.
    J Mol Biol. 2010 Jun 11;399(3):501-11 PMID: 20417217
  100. Time-resolved surface charge change on the cytoplasmic side of bacteriorhodopsin.
    FEBS Lett. 1995 Oct 2;373(1):81-4 PMID: 7589439
  101. Electrical spiking in Escherichia coli probed with a fluorescent voltage-indicating protein.
    Science. 2011 Jul 15;333(6040):345-8 PMID: 21764748
  102. Elucidation of the nature of the conformational changes of the EF-interhelical loop in bacteriorhodopsin and of the helix VIII on the cytoplasmic surface of bovine rhodopsin: a time-resolved fluorescence depolarization study.
    J Mol Biol. 2003 May 2;328(3):705-19 PMID: 12706727
  103. Photoisomerization in proteorhodopsin mutant D97N.
    Photochem Photobiol. 2007 Mar-Apr;83(2):226-31 PMID: 16808594
  104. Arrestin-rhodopsin binding stoichiometry in isolated rod outer segment membranes depends on the percentage of activated receptors.
    J Biol Chem. 2011 Mar 4;286(9):7359-69 PMID: 21169358
  105. Light-induced changes in the structure and accessibility of the cytoplasmic loops of rhodopsin in the activated MII state.
    Biochemistry. 2002 Jun 25;41(25):7875-84 PMID: 12069576
  106. Sequence of interactions in receptor-G protein coupling.
    J Biol Chem. 2004 Jun 4;279(23):24283-90 PMID: 15007073
  107. Potent peptide analogues of a G protein receptor-binding region obtained with a combinatorial library.
    J Biol Chem. 1996 Jan 5;271(1):361-6 PMID: 8550587
  108. Transducin activation by nanoscale lipid bilayers containing one and two rhodopsins.
    J Biol Chem. 2007 May 18;282(20):14875-81 PMID: 17395586
  109. Structural changes in bacteriorhodopsin during proton translocation revealed by neutron diffraction.
    Proc Natl Acad Sci U S A. 1989 Oct;86(20):7876-9 PMID: 2554293
  110. Atomic-force microscopy: Rhodopsin dimers in native disc membranes.
    Nature. 2003 Jan 9;421(6919):127-8 PMID: 12520290
  111. Effect of packing density on rhodopsin stability and function in polyunsaturated membranes.
    Biophys J. 2005 Sep;89(3):1833-40 PMID: 15980173
  112. Monitoring the interaction of a single G-protein key binding site with rhodopsin disk membranes upon light activation.
    Biochemistry. 2009 May 12;48(18):3801-3 PMID: 19301833
  113. Light-induced conformational changes of rhodopsin probed by fluorescent alexa594 immobilized on the cytoplasmic surface.
    Biochemistry. 2000 Dec 12;39(49):15225-33 PMID: 11106502
  114. Probing rhodopsin-transducin interactions by surface modification and mass spectrometry.
    Biochemistry. 2004 Sep 7;43(35):11153-62 PMID: 15366925
  115. Protein catalysis of the retinal subpicosecond photoisomerization in the primary process of bacteriorhodopsin photosynthesis.
    Science. 1993 Aug 13;261(5123):891-4 PMID: 17783735
  116. What site-directed labeling studies tell us about the mechanism of rhodopsin activation and G-protein binding.
    Photochem Photobiol Sci. 2010 Nov;9(11):1466-74 PMID: 20967340
  117. Covalently bound pH-indicator dyes at selected extracellular or cytoplasmic sites in bacteriorhodopsin. 1. Proton migration along the surface of bacteriorhodopsin micelles and its delayed transfer from surface to bulk.
    Biochemistry. 1994 Nov 22;33(46):13684-92 PMID: 7947777
  118. Photosensory functions of channelrhodopsins in native algal cells.
    Photochem Photobiol. 2009 Mar-Apr;85(2):556-63 PMID: 19222796
  119. Fluorescent PET (photoinduced electron transfer) sensors as potent analytical tools.
    Analyst. 2009 Dec;134(12):2385-93 PMID: 19918605
  120. Opsin is present as dimers in COS1 cells: identification of amino acids at the dimeric interface.
    Proc Natl Acad Sci U S A. 2006 Feb 28;103(9):3054-9 PMID: 16492774
  121. Structural changes in bacteriorhodopsin caused by two-photon-induced photobleaching.
    J Phys Chem B. 2012 Jun 28;116(25):7455-62 PMID: 22512248
  122. Interaction of a two-transmembrane-helix peptide with lipid bilayers and dodecyl sulfate micelles.
    Biophys Chem. 2011 Dec;159(2-3):321-7 PMID: 21924540
  123. Light-regulated binding of proteins to photoreceptor membranes and its use for the purification of several rod cell proteins.
    Methods Enzymol. 1982;81:556-64 PMID: 6124869
  124. Surface-bound optical probes monitor protein translocation and surface potential changes during the bacteriorhodopsin photocycle.
    Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):5996-6000 PMID: 1497755
  125. Characterization of membrane protein non-native states. 2. The SDS-unfolded states of rhodopsin.
    Biochemistry. 2010 Aug 3;49(30):6329-40 PMID: 20575562
  126. Determination of protein secondary structure and solvent accessibility using site-directed fluorescence labeling. Studies of T4 lysozyme using the fluorescent probe monobromobimane.
    Biochemistry. 1999 Dec 7;38(49):16383-93 PMID: 10587464
  127. Distance mapping in proteins using fluorescence spectroscopy: the tryptophan-induced quenching (TrIQ) method.
    Biochemistry. 2010 Nov 16;49(45):9722-31 PMID: 20886836
  128. Monomeric rhodopsin is sufficient for normal rhodopsin kinase (GRK1) phosphorylation and arrestin-1 binding.
    J Biol Chem. 2011 Jan 14;286(2):1420-8 PMID: 20966068
  129. Channelrhodopsin-1: a light-gated proton channel in green algae.
    Science. 2002 Jun 28;296(5577):2395-8 PMID: 12089443
  130. Fluorescence of crayfish metarhodopsin studied in single rhabdoms.
    Biophys J. 1981 Sep;35(3):653-64 PMID: 7272455
  131. Functions of a new photoreceptor membrane.
    Proc Natl Acad Sci U S A. 1973 Oct;70(10):2853-7 PMID: 4517939
  132. Coupling ligand structure to specific conformational switches in the beta2-adrenoceptor.
    Nat Chem Biol. 2006 Aug;2(8):417-22 PMID: 16799554
  133. Monomeric G protein-coupled receptor rhodopsin in solution activates its G protein transducin at the diffusion limit.
    Proc Natl Acad Sci U S A. 2007 Jun 26;104(26):10859-64 PMID: 17578920
  134. Crystal structure of opsin in its G-protein-interacting conformation.
    Nature. 2008 Sep 25;455(7212):497-502 PMID: 18818650
  135. Halorhodopsin: a light-driven chloride ion pump.
    Annu Rev Biophys Biophys Chem. 1986;15:11-28 PMID: 2424472
  136. Efficiencies of activation of transducin by cone and rod visual pigments.
    Biochemistry. 2013 Apr 30;52(17):3010-8 PMID: 23570417
  137. Excited-state dynamics of bacteriorhodopsin.
    Biophys J. 1985 Jan;47(1):43-54 PMID: 3978189
  138. Surface charge changes upon formation of the signaling state in visual rhodopsin.
    Photochem Photobiol. 2009 Mar-Apr;85(2):501-8 PMID: 19222792
  139. Dissection of environmental changes at the cytoplasmic surface of light-activated bacteriorhodopsin and visual rhodopsin: sequence of spectrally silent steps.
    Photochem Photobiol. 2009 Mar-Apr;85(2):570-7 PMID: 19222795
  140. Visual arrestin binding to rhodopsin. Diverse functional roles of positively charged residues within the phosphorylation-recognition region of arrestin.
    J Biol Chem. 1995 Mar 17;270(11):6010-6 PMID: 7890732
Article Info
Journal
Biochimica et biophysica acta
Abbr.
Biochim Biophys Acta
ISSN
0006-3002
DOI
10.1016/j.bbabio.2013.10.008
Email
alexiev@physik.fu-berlin.de
Published
2014-05-00
Epub
2013-00-29
Pages
694-709
Language
English
Region
Netherlands
NLM ID
0217513
PMCID
PMC3965647
Subset
IM
Grants
NEI NIH HHS · R01 EY015436 · United States
External Links
PubMed source DOI: 10.1016/j.bbabio.2013.10.008 PMC Full Text
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