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PMID: 22010969 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Ultrasensitive measurements of microbial rhodopsin photocycles using photochromic FRET.

Photochemistry and photobiology ·Vol. 88 ·No. 1 ·2012-00-00 ·Pages 90-7

Bayraktar H, Fields AP, Kralj JM, Spudich JL, Rothschild KJ, Cohen AE

Abstract

Microbial rhodopsins are an important class of light-activated transmembrane proteins whose function is typically studied on bulk samples. Herein, we apply photochromic fluorescence resonance energy transfer to investigate the dynamics of these proteins with sensitivity approaching the single-molecule limit. The brightness of a covalently linked organic fluorophore is modulated by changes in the absorption spectrum of the endogenous retinal chromophore that occur as the molecule undergoes a light-activated photocycle. We studied the photocycles of blue-absorbing proteorhodopsin and sensory rhodopsin II (SRII). Clusters of 2-3 molecules of SRII clearly showed a light-induced photocycle. Single molecules of SRII showed a photocycle upon signal averaging over several illumination cycles.

MeSH Terms
Fluorescence Resonance Energy Transfer Photochemistry Rhodopsin/physiology
Chemicals
Rhodopsin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Bayraktar Halil
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.
Fields Alexander P
Kralj Joel M
Spudich John L
Rothschild Kenneth J
Cohen Adam E
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Article Info
Journal
Photochemistry and photobiology
Abbr.
Photochem Photobiol
ISSN
1751-1097
DOI
10.1111/j.1751-1097.2011.01011.x
Published
2012-00-00
Epub
2011-00-17
Pages
90-7
Language
English
Region
United States
NLM ID
0376425
PMCID
PMC3253248
Subset
IM
Grants
NIGMS NIH HHS · R01 GM069969 · United States
NIGMS NIH HHS · R01GM069969 · United States
NIGMS NIH HHS · R37GM027750 · United States
NIH HHS · DP2 OD007428-01 · United States
NIBIB NIH HHS · R01 EB012498 · United States
NIBIB NIH HHS · R01 EB012498-01 · United States
NIGMS NIH HHS · R01 GM069969-05 · United States
NIH HHS · DP2 OD007428 · United States
NIGMS NIH HHS · R37 GM027750 · United States
External Links
PubMed source DOI: 10.1111/j.1751-1097.2011.01011.x PMC Full Text View journal details
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