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PMID: 17069872 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Arrestin can act as a regulator of rhodopsin photochemistry.

Vision research ·Vol. 46 ·No. 27 ·2006-12-00 ·Pages 4532-46

Sommer ME, Farrens DL

Abstract

We report that visual arrestin can regulate retinal release and late photoproduct formation in rhodopsin. Our experiments, which employ a fluorescently labeled arrestin and rhodopsin solubilized in detergent/phospholipid micelles, indicate that arrestin can trap a population of retinal in the binding pocket with an absorbance characteristic of Meta II with the retinal Schiff-base intact. Furthermore, arrestin can convert Metarhodopsin III (formed either by thermal decay or blue-light irradiation) to a Meta II-like absorbing species. Together, our results suggest arrestin may be able to play a more complex role in the rod cell besides simply quenching transducin activity. This possibility may help explain why arrestin deficiency leads to problems like stationary night blindness (Oguchi disease) and retinal degeneration.

MeSH Terms
Animals Arrestin/analysis,physiology Dark Adaptation Micelles Night Blindness/metabolism Protein Binding Recombinant Proteins/analysis,metabolism Retinal Rod Photoreceptor Cells/metabolism Retinaldehyde/metabolism Rhodopsin/metabolism Schiff Bases/metabolism Spectrophotometry, Ultraviolet Transducin/metabolism Vision, Ocular
Chemicals
Arrestin Micelles Recombinant Proteins Schiff Bases metarhodopsins Rhodopsin Transducin Retinaldehyde
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sommer Martha E
Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, OR 97239-3098, USA.
Farrens David L
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Article Info
Journal
Vision research
Abbr.
Vision Res
ISSN
0042-6989
DOI
10.1016/j.visres.2006.08.031
Published
2006-12-00
Epub
2006-00-27
Pages
4532-46
Language
English
Region
England
NLM ID
0417402
PMCID
PMC2877124
Subset
IM
Grants
NIDA NIH HHS · R01 DA018169 · United States
NEI NIH HHS · R01 EY015436 · United States
NIDA NIH HHS · DA018169 · United States
NEI NIH HHS · EY015436 · United States
External Links
PubMed source DOI: 10.1016/j.visres.2006.08.031 PMC Full Text View journal details
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