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PMID: 2410905 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning of the genome of a cardiotropic Coxsackie B3 virus: full-length reverse-transcribed recombinant cDNA generates infectious virus in mammalian cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 14 ·1985-07-00 ·Pages 4818-22

Kandolf R, Hofschneider PH

Abstract

The molecular cloning of double-stranded cDNA synthesized from the single-stranded RNA genome of the cardiotropic Coxsackie B3 virus (Nancy strain) is reported. Full-length reverse-transcribed cloned viral cDNA of approximately equal to 7500 nucleotides generated infectious antigenically identical Coxsackie B3 virus upon transfection of recombinant plasmid DNA into mammalian cells, demonstrating the molecular cloning of a biologically active viral cDNA copy. Furthermore, the cloned cDNA is characterized by restriction enzyme analysis and partial nucleotide sequencing of the 5' end. The Coxsackie B3 virus cDNA described can now be used to study the molecular basis of human enteroviral heart disease, and it provides a valuable diagnostic means for patients with suspected viral heart disease.

MeSH Terms

Animals Base Sequence Cell Line Chlorocebus aethiops Cloning, Molecular Coxsackievirus Infections/microbiology DNA/metabolism DNA Restriction Enzymes Enterovirus/genetics Genes, Viral Kidney Myocarditis/microbiology Nucleic Acid Hybridization RNA, Viral/genetics,isolation & purification RNA-Directed DNA Polymerase/metabolism Transfection

Chemicals

RNA, Viral DNA RNA-Directed DNA Polymerase DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kandolf R

Hofschneider P H

References (25)

25 references, click to expand

Replicase gene of coxsackievirus B3.
J Virol. 1984 Sep;51(3):742-6 PMID: 6088796
Detection of enteroviruses by spot hybridization.
J Clin Microbiol. 1984 Mar;19(3):436-8 PMID: 6325492
A new technique for the assay of infectivity of human adenovirus 5 DNA.
Virology. 1973 Apr;52(2):456-67 PMID: 4705382
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
An improved technique for obtaining enhanced infectivity with herpes simplex virus type 1 DNA.
J Gen Virol. 1976 Dec;33(3):447-58 PMID: 187721
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 PMID: 73185
Sequence of picornavirus RNAs containing a radioiodinated 5'-linked peptide reveals a conserved 5' sequence.
Proc Natl Acad Sci U S A. 1980 Jan;77(1):303-7 PMID: 6244553
Sequencing end-labeled DNA with base-specific chemical cleavages.
Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
Cloning of cDNA of major antigen of foot and mouth disease virus and expression in E. coli.
Nature. 1981 Feb 12;289(5798):555-9 PMID: 6258084
Picornaviral structure and assembly.
Microbiol Rev. 1981 Jun;45(2):287-315 PMID: 7022155
Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome.
Proc Natl Acad Sci U S A. 1981 Aug;78(8):4887-91 PMID: 6272282
Cloned poliovirus complementary DNA is infectious in mammalian cells.
Science. 1981 Nov 20;214(4523):916-9 PMID: 6272391
Molecular cloning of the genome of poliovirus type 1.
Proc Natl Acad Sci U S A. 1981 Oct;78(10):5983-7 PMID: 6273836
High-efficiency cloning of full-length cDNA.
Mol Cell Biol. 1982 Feb;2(2):161-70 PMID: 6287227
Dilated cardiomyopathies of the adult (second of two parts).
N Engl J Med. 1982 Oct 28;307(18):1119-26 PMID: 6750401
High frequency of antigenic variants among naturally occurring human Coxsackie B4 virus isolates identified by monoclonal antibodies.
Nature. 1982 Nov 25;300(5890):374-6 PMID: 6183593
Poliovirus type 3: molecular cloning of the genome and nucleotide sequence of the region encoding the protease and polymerase proteins.
Nucleic Acids Res. 1983 Mar 11;11(5):1267-81 PMID: 6298739
Isolation and characterization of a membrane-bound population of group B coxsackieviruses.
J Virol. 1983 Feb;45(2):832-41 PMID: 6300437
Studies on transformation of Escherichia coli with plasmids.
J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
Molecular cloning and characterization of hepatitis A virus cDNA.
Proc Natl Acad Sci U S A. 1983 Oct;80(19):5885-9 PMID: 6310601
Portraits of viruses: the picornaviruses.
Intervirology. 1983;20(2-3):61-100 PMID: 6313547
A simple and very efficient method for generating cDNA libraries.
Gene. 1983 Nov;25(2-3):263-9 PMID: 6198242
Coxsackie B3 virus can replicate in cultured human foetal heart cells and is inhibited by interferon.
J Mol Cell Cardiol. 1985 Feb;17(2):167-81 PMID: 3889351

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.82.14.4818

Published

1985-07-00

Pages

4818-22

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC390996

Subset

Databases

GENBANK

M11232

External Links

PubMed source DOI: 10.1073/pnas.82.14.4818 PMC Full Text View journal details

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Molecular cloning of the genome of a cardiotropic Coxsackie B3 virus: full-length reverse-transcribed recombinant cDNA generates infectious virus in mammalian cells.

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