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PMID: 23918260 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

OsbZIP71, a bZIP transcription factor, confers salinity and drought tolerance in rice.

Plant molecular biology ·Vol. 84 ·No. 1-2 ·2014-01-00 ·Pages 19-36

Liu C, Mao B, Ou S, Wang W, Liu L, Wu Y, Chu C, Wang X

Abstract

The bZIP transcription factor (TF) family plays an important role in the abscisic acid (ABA) signaling pathway of abiotic stress in plants. We here report the cloning and characterization of OsbZIP71, which encodes a rice bZIP TF. Functional analysis showed that OsbZIP71 is a nuclear-localized protein that specifically binds to the G-box motif, but has no transcriptional activity both in yeast and rice protoplasts. In yeast two-hybrid assays, OsbZIP71 can form both homodimers and heterodimers with Group C members of the bZIP gene family. Expression of OsbZIP71 was strongly induced by drought, polyethylene glycol (PEG), and ABA treatments, but repressed by salt treatment. OsbZIP71 overexpressing (p35S::OsbZIP71) rice significantly improved tolerance to drought, salt and PEG osmotic stresses. In contrast, RNAi knockdown transgenic lines were much more sensitive to salt, PEG osmotic stresses, and also ABA treatment. Inducible expression (RD29A::OsbZIP71) lines were significantly improved their tolerance to PEG osmotic stresses, but hypersensitivity to salt, and insensitivity to ABA. Real-time PCR analysis revealed that the abiotic stress-related genes, OsVHA-B, OsNHX1, COR413-TM1, and OsMyb4, were up-regulated in overexpressing lines, while these same genes were down-regulated in RNAi lines. Chromatin immunoprecipitation analysis confirmed that OsbZIP71 directly binds the promoters of OsNHX1 and COR413-TM1 in vivo. These results suggest that OsbZIP71 may play an important role in ABA-mediated drought and salt tolerance in rice.

MeSH Terms
Abscisic Acid Droughts Germination Oryza/genetics,metabolism Phylogeny Plant Proteins/genetics,metabolism Plants, Genetically Modified Protein Binding Protein Transport Salinity Seeds/genetics,physiology Stress, Physiological Transcription Factors/genetics,metabolism Water/metabolism
Chemicals
Plant Proteins Transcription Factors Water Abscisic Acid
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Liu Citao
State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Mao Bigang
Ou Shujun
Wang Wei
Liu Linchuan
Wu Yanbin
Chu Chengcai
Wang Xiping
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
1573-5028
DOI
10.1007/s11103-013-0115-3
Published
2014-01-00
Epub
2013-00-06
Pages
19-36
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Corrections
ErratumIn
PMID: 29946804
External Links
PubMed source DOI: 10.1007/s11103-013-0115-3 View journal details
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