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PMID: 22189955 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

bZIP transcription factor OsbZIP52/RISBZ5: a potential negative regulator of cold and drought stress response in rice.

Planta ·Vol. 235 ·No. 6 ·2012-06-00 ·Pages 1157-69

Liu C, Wu Y, Wang X

Abstract

OsbZIP52/RISBZ5 is a member of the basic leucine zipper (bZIP) transcription factor (TF) family in rice (Oryza sativa) isolated from rice (Zhonghua11) panicles. Expression of the OsbZIP52 gene was strongly induced by low temperature (4°C) but not by drought, PEG, salt, or ABA. The subcellular localization of OsbZIP52-GFP in onion (Allium cepa) epidermis cells revealed that OsbZIP52 is a nuclear localized protein. A transactivation assay in yeast demonstrated that OsbZIP52 functions as a transcriptional activator and can specifically bind to the G-box promoter motif. In a yeast two-hybrid (Y-2-H) experiment, OsbZIP52 was able to form homodimeric complexes. Rice plants overexpressing OsbZIP52 showed significantly increased sensitivity to cold and drought stress. Real-time PCR analysis revealed that some abiotic stress-related genes, such as OsLEA3, OsTPP1, Rab25, gp1 precursor, β-gal, LOC_Os05g11910 and LOC_Os05g39250, were down-regulated in OsbZIP52 overexpression lines. These results suggest that OsbZIP52/RISBZ5 could function as a negative regulator in cold and drought stress environments.

MeSH Terms
Abscisic Acid/pharmacology Amino Acid Sequence Basic-Leucine Zipper Transcription Factors/chemistry,genetics,metabolism Biological Assay Cell Nucleus/drug effects,metabolism Cold Temperature Down-Regulation/drug effects,genetics Droughts Gene Expression Profiling Gene Expression Regulation, Plant/drug effects Molecular Sequence Data Nucleotide Motifs/genetics Oryza/drug effects,genetics,physiology Phylogeny Plant Proteins/chemistry,genetics,metabolism Promoter Regions, Genetic/genetics Protein Binding/drug effects Protein Multimerization/drug effects Saccharomyces cerevisiae/metabolism Seedlings/drug effects,genetics Stress, Physiological/drug effects,genetics Transcriptional Activation/drug effects,genetics
Chemicals
Basic-Leucine Zipper Transcription Factors Plant Proteins Abscisic Acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Liu Citao
Key Laboratory of Cell Proliferation and Regulation of Ministry of Education, College of Life Sciences, Beijing Normal University, Beijing 100875, People's Republic of China.
Wu Yanbin
Wang Xiping
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Article Info
Journal
Planta
Abbr.
Planta
ISSN
1432-2048
DOI
10.1007/s00425-011-1564-z
Published
2012-06-00
Epub
2011-00-22
Pages
1157-69
Language
English
Region
Germany
NLM ID
1250576
Subset
IM
External Links
PubMed source DOI: 10.1007/s00425-011-1564-z View journal details
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