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PMID: 9049302 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Rapid stimulation of a soybean protein-serine kinase that phosphorylates a novel bZIP DNA-binding protein, G/HBF-1, during the induction of early transcription-dependent defenses.

The EMBO journal ·Vol. 16 ·No. 4 ·1997-02-17 ·Pages 726-38

Dröge-Laser W, Kaiser A, Lindsay WP, Halkier BA, Loake GJ, Doerner P, Dixon RA, Lamb C

Abstract

The G-box (CACGTG) and H-box (CCTACC) cis elements function in the activation of phenylpropanoid biosynthetic genes involved in the elaboration of lignin precursors, phytoalexins and the secondary signal salicylic acid as early responses to pathogen attack. We have isolated a soybean cDNA encoding a novel bZIP protein, G/HBF-1, which binds to both the G-box and adjacent H-box in the proximal region of the chalcone synthase chs15 promoter. While G/HBF-1 transcript and protein levels do not increase during the induction of phenylpropanoid biosynthetic genes, G/HBF-1 is phosphorylated rapidly in elicited soybean cells, almost exclusively on serine residues. Using recombinant G/HBF-1 as a substrate, we identified a cytosolic protein-serine kinase that is rapidly and transiently stimulated in cells elicited with either glutathione or an avirulent strain of the soybean pathogen Pseudomonas syringae pv. glycinea. Phosphorylation of G/HBF-1 in vitro enhances binding to the chs15 promoter and we conclude that stimulation of G/HBF-1 kinase activity and G/HBF-1 phosphorylation are terminal events in a signal pathway for activation of early transcription-dependent plant defense responses.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Complementary/genetics DNA, Plant/genetics,metabolism DNA-Binding Proteins/genetics,metabolism Enzyme Activation Gene Expression Regulation, Plant/physiology Glutathione/pharmacology Leucine Zippers/genetics Molecular Sequence Data Phosphorylation Plant Proteins Promoter Regions, Genetic/genetics Protein Serine-Threonine Kinases/metabolism Pseudomonas/physiology RNA, Messenger/biosynthesis Recombinant Fusion Proteins Soybeans/enzymology,physiology Transcription Factors/genetics,metabolism Transcription, Genetic
Chemicals
DNA, Complementary DNA, Plant DNA-Binding Proteins G-HBF-1 protein, Glycine max Plant Proteins RNA, Messenger Recombinant Fusion Proteins Transcription Factors Protein Serine-Threonine Kinases Glutathione
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Dröge-Laser W
Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Kaiser A
Lindsay W P
Halkier B A
Loake G J
Doerner P
Dixon R A
Lamb C
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/16.4.726
Published
1997-02-17
Pages
726-38
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1169674
Subset
IM
Databases
GENBANK
Y10685
External Links
PubMed source DOI: 10.1093/emboj/16.4.726 PMC Full Text View journal details
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