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PMID: 20487379 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

ABO3, a WRKY transcription factor, mediates plant responses to abscisic acid and drought tolerance in Arabidopsis.

The Plant journal : for cell and molecular biology ·Vol. 63 ·No. 3 ·2010-08-00 ·Pages 417-29

Ren X, Chen Z, Liu Y, Zhang H, Zhang M, Liu Q, Hong X, Zhu JK, Gong Z

Abstract

The biological functions of WRKY transcription factors in plants have been widely studied, but their roles in abiotic stress are still not well understood. We isolated an ABA overly sensitive mutant, abo3, which is disrupted by a T-DNA insertion in At1g66600 encoding a WRKY transcription factor AtWRKY63. The mutant was hypersensitive to ABA in both seedling establishment and seedling growth. However, stomatal closure was less sensitive to ABA, and the abo3 mutant was less drought tolerant than the wild type. Northern blot analysis indicated that the expression of the ABA-responsive transcription factor ABF2/AREB1 was markedly lower in the abo3 mutant than in the wild type. The abo3 mutation also reduced the expression of stress-inducible genes RD29A and COR47, especially early during ABA treatment. ABO3 is able to bind the W-box in the promoter of ABF2in vitro. These results uncover an important role for a WRKY transcription factor in plant responses to ABA and drought stress.

Keywords
Arabidopsis WRKY transcription factor abscisic acid drought stress
MeSH Terms
Adaptation, Physiological/physiology Arabidopsis/genetics,metabolism,physiology Arabidopsis Proteins/genetics,physiology Blotting, Northern Droughts Genes, Plant Mutation Plant Stomata/physiology Stress, Physiological
Chemicals
Arabidopsis Proteins
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Ren Xiaozhi
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, ChinaDepartment of Botany and Plant Sciences, Institute for Integrative Genome Biology, 2150 Batchelor Hall, University of California, Riverside, CA 92521, USAChina Agricultural University, University of California-Riverside Center for Biological Sciences and Biotechnology, Beijing 100193, ChinaNational Center for Plant Gene Research, Beijing 100193, China.
Chen Zhizhong
Liu Yue
Zhang Hairong
Zhang Min
Liu Qian
Hong Xuhui
Zhu Jian-Kang
Gong Zhizhong
References (75)
75 references, click to expand
  1. The ABSCISIC ACID-INSENSITIVE3, FUSCA3, and LEAFY COTYLEDON1 loci act in concert to control multiple aspects of Arabidopsis seed development.
    Plant Cell. 1997 Aug;9(8):1265-77 PMID: 9286105
  2. Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response.
    Plant Mol Biol. 2003 Jan;51(1):21-37 PMID: 12602888
  3. Identification of a cis-regulatory region of a gene in Arabidopsis thaliana whose induction by dehydration is mediated by abscisic acid and requires protein synthesis.
    Mol Gen Genet. 1995 May 20;247(4):391-8 PMID: 7770045
  4. Epigenetic regulation, somatic homologous recombination, and abscisic acid signaling are influenced by DNA polymerase epsilon mutation in Arabidopsis.
    Plant Cell. 2009 Feb;21(2):386-402 PMID: 19244142
  5. The WRKY superfamily of plant transcription factors.
    Trends Plant Sci. 2000 May;5(5):199-206 PMID: 10785665
  6. ABI1 of Arabidopsis is a protein serine/threonine phosphatase highly regulated by the proton and magnesium ion concentration.
    FEBS Lett. 1998 Mar 6;424(1-2):100-4 PMID: 9537523
  7. Arabidopsis basic leucine zipper proteins that mediate stress-responsive abscisic acid signaling.
    Plant Cell. 2002 Feb;14(2):343-57 PMID: 11884679
  8. The combined effect of drought stress and heat shock on gene expression in tobacco.
    Plant Physiol. 2002 Nov;130(3):1143-51 PMID: 12427981
  9. Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis.
    Proc Natl Acad Sci U S A. 2009 Oct 13;106(41):17588-93 PMID: 19805022
  10. Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins.
    Science. 2009 May 22;324(5930):1068-71 PMID: 19407142
  11. AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis.
    Plant Cell. 2005 Dec;17(12):3470-88 PMID: 16284313
  12. A novel drought-inducible gene, ATAF1, encodes a NAC family protein that negatively regulates the expression of stress-responsive genes in Arabidopsis.
    Plant Mol Biol. 2007 Jan;63(2):289-305 PMID: 17031511
  13. Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit.
    Proc Natl Acad Sci U S A. 1997 Feb 4;94(3):1035-40 PMID: 9023378
  14. Annotations and functional analyses of the rice WRKY gene superfamily reveal positive and negative regulators of abscisic acid signaling in aleurone cells.
    Plant Physiol. 2005 Jan;137(1):176-89 PMID: 15618416
  15. ABI2, a second protein phosphatase 2C involved in abscisic acid signal transduction in Arabidopsis.
    FEBS Lett. 1998 Jan 16;421(3):185-90 PMID: 9468303
  16. Cold acclimation in Arabidopsis and wheat : a response associated with expression of related genes encoding ;boiling-stable' polypeptides.
    Plant Physiol. 1990 Nov;94(3):1078-83 PMID: 16667799
  17. Regulation of Arabidopsis thaliana Em genes: role of ABI5.
    Plant J. 2002 May;30(3):373-83 PMID: 12000684
  18. Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray.
    Plant J. 2002 Aug;31(3):279-92 PMID: 12164808
  19. Role of farnesyltransferase in ABA regulation of guard cell anion channels and plant water loss.
    Science. 1998 Oct 9;282(5387):287-90 PMID: 9765153
  20. WRKY transcription factors: from DNA binding towards biological function.
    Curr Opin Plant Biol. 2004 Oct;7(5):491-8 PMID: 15337090
  21. ABFs, a family of ABA-responsive element binding factors.
    J Biol Chem. 2000 Jan 21;275(3):1723-30 PMID: 10636868
  22. WRKY75 transcription factor is a modulator of phosphate acquisition and root development in Arabidopsis.
    Plant Physiol. 2007 Apr;143(4):1789-801 PMID: 17322336
  23. TRANSPARENT TESTA GLABRA2, a trichome and seed coat development gene of Arabidopsis, encodes a WRKY transcription factor.
    Plant Cell. 2002 Jun;14(6):1359-75 PMID: 12084832
  24. Abscisic acid-dependent multisite phosphorylation regulates the activity of a transcription activator AREB1.
    Proc Natl Acad Sci U S A. 2006 Feb 7;103(6):1988-93 PMID: 16446457
  25. The Arabidopsis trehalose-6-P synthase AtTPS1 gene is a regulator of glucose, abscisic acid, and stress signaling.
    Plant Physiol. 2004 Nov;136(3):3649-59 PMID: 15516499
  26. Characterization of a Cold-Regulated Wheat Gene Related to Arabidopsis cor47.
    Plant Physiol. 1992 Oct;100(2):915-22 PMID: 16653076
  27. A novel WRKY transcription factor, SUSIBA2, participates in sugar signaling in barley by binding to the sugar-responsive elements of the iso1 promoter.
    Plant Cell. 2003 Sep;15(9):2076-92 PMID: 12953112
  28. Modulation of abscisic acid signal transduction and biosynthesis by an Sm-like protein in Arabidopsis.
    Dev Cell. 2001 Dec;1(6):771-81 PMID: 11740939
  29. The Arabidopsis abscisic acid response locus ABI4 encodes an APETALA 2 domain protein.
    Plant Cell. 1998 Jun;10(6):1043-54 PMID: 9634591
  30. A new member of the Arabidopsis WRKY transcription factor family, AtWRKY6, is associated with both senescence- and defence-related processes.
    Plant J. 2001 Oct;28(2):123-33 PMID: 11722756
  31. A plant leucine zipper protein that recognizes an abscisic acid response element.
    Science. 1990 Oct 12;250(4978):267-71 PMID: 2145628
  32. ABI5 acts downstream of ABI3 to execute an ABA-dependent growth arrest during germination.
    Plant J. 2002 Nov;32(3):317-28 PMID: 12410810
  33. ROS1, a repressor of transcriptional gene silencing in Arabidopsis, encodes a DNA glycosylase/lyase.
    Cell. 2002 Dec 13;111(6):803-14 PMID: 12526807
  34. Identification of AtHD2C as a novel regulator of abscisic acid responses in Arabidopsis.
    Plant J. 2006 Apr;46(1):124-33 PMID: 16553900
  35. The WRKY70 transcription factor of Arabidopsis influences both the plant senescence and defense signaling pathways.
    Planta. 2007 Jun;226(1):125-37 PMID: 17310369
  36. Three Arabidopsis SnRK2 protein kinases, SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1 and SRK2I/SnRK2.3, involved in ABA signaling are essential for the control of seed development and dormancy.
    Plant Cell Physiol. 2009 Jul;50(7):1345-63 PMID: 19541597
  37. ABF2, an ABRE-binding bZIP factor, is an essential component of glucose signaling and its overexpression affects multiple stress tolerance.
    Plant J. 2004 Oct;40(1):75-87 PMID: 15361142
  38. A gateway cloning vector set for high-throughput functional analysis of genes in planta.
    Plant Physiol. 2003 Oct;133(2):462-9 PMID: 14555774
  39. The transcription factors HvABI5 and HvVP1 are required for the abscisic acid induction of gene expression in barley aleurone cells.
    Plant Cell. 2003 Jan;15(1):271-84 PMID: 12509536
  40. Constitutive expression of abiotic stress-inducible hot pepper CaXTH3, which encodes a xyloglucan endotransglucosylase/hydrolase homolog, improves drought and salt tolerance in transgenic Arabidopsis plants.
    FEBS Lett. 2006 May 29;580(13):3136-44 PMID: 16684525
  41. Arabidopsis mutant deficient in 3 abscisic acid-activated protein kinases reveals critical roles in growth, reproduction, and stress.
    Proc Natl Acad Sci U S A. 2009 May 19;106(20):8380-5 PMID: 19420218
  42. Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions.
    Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11632-7 PMID: 11005831
  43. A negative regulator encoded by a rice WRKY gene represses both abscisic acid and gibberellins signaling in aleurone cells.
    Plant Mol Biol. 2009 May;70(1-2):139-51 PMID: 19199048
  44. The plant hormone abscisic acid mediates the drought-induced expression but not the seed-specific expression of rd22, a gene responsive to dehydration stress in Arabidopsis thaliana.
    Mol Gen Genet. 1993 Apr;238(1-2):17-25 PMID: 8479424
  45. Salt and drought stress signal transduction in plants.
    Annu Rev Plant Biol. 2002;53:247-73 PMID: 12221975
  46. Arabidopsis CBF1 overexpression induces COR genes and enhances freezing tolerance.
    Science. 1998 Apr 3;280(5360):104-6 PMID: 9525853
  47. The Viviparous-1 developmental gene of maize encodes a novel transcriptional activator.
    Cell. 1991 Sep 6;66(5):895-905 PMID: 1889090
  48. Modular nature of abscisic acid (ABA) response complexes: composite promoter units that are necessary and sufficient for ABA induction of gene expression in barley.
    Plant Cell. 1996 Jul;8(7):1107-19 PMID: 8768371
  49. A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress.
    Plant Cell. 1994 Feb;6(2):251-64 PMID: 8148648
  50. Homeodomain protein ATHB6 is a target of the protein phosphatase ABI1 and regulates hormone responses in Arabidopsis.
    EMBO J. 2002 Jun 17;21(12):3029-38 PMID: 12065416
  51. The Arabidopsis cold-responsive transcriptome and its regulation by ICE1.
    Plant Cell. 2005 Nov;17(11):3155-75 PMID: 16214899
  52. Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis.
    Plant Cell. 1998 Aug;10(8):1391-406 PMID: 9707537
  53. Early nuclear events in plant defence signalling: rapid gene activation by WRKY transcription factors.
    EMBO J. 1999 Sep 1;18(17):4689-99 PMID: 10469648
  54. Arabidopsis MEKK1 can take a short cut: it can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter.
    Plant Mol Biol. 2007 Sep;65(1-2):63-76 PMID: 17587183
  55. Cloning and characterization of a cold- and ABA-inducible Arabidopsis gene.
    Plant Mol Biol. 1990 Jul;15(1):137-44 PMID: 2151730
  56. A dehydration-induced NAC protein, RD26, is involved in a novel ABA-dependent stress-signaling pathway.
    Plant J. 2004 Sep;39(6):863-76 PMID: 15341629
  57. Regulators of PP2C phosphatase activity function as abscisic acid sensors.
    Science. 2009 May 22;324(5930):1064-8 PMID: 19407143
  58. Role of an Arabidopsis AP2/EREBP-type transcriptional repressor in abscisic acid and drought stress responses.
    Plant Cell. 2005 Aug;17(8):2384-96 PMID: 15994908
  59. Physical and functional interactions between pathogen-induced Arabidopsis WRKY18, WRKY40, and WRKY60 transcription factors.
    Plant Cell. 2006 May;18(5):1310-26 PMID: 16603654
  60. Viviparous1 alters global gene expression patterns through regulation of abscisic acid signaling.
    Plant Physiol. 2003 Jul;132(3):1664-77 PMID: 12857845
  61. Functional characterization of Arabidopsis NaCl-inducible WRKY25 and WRKY33 transcription factors in abiotic stresses.
    Plant Mol Biol. 2009 Jan;69(1-2):91-105 PMID: 18839316
  62. Promoters from kin1 and cor6.6, two Arabidopsis thaliana low-temperature- and ABA-inducible genes, direct strong beta-glucuronidase expression in guard cells, pollen and young developing seeds.
    Plant Mol Biol. 1995 Jul;28(4):619-34 PMID: 7647295
  63. The WRKY family of transcription factors in rice and Arabidopsis and their origins.
    DNA Res. 2005 Feb 28;12(1):9-26 PMID: 16106749
  64. Reactive oxygen signaling and abiotic stress.
    Physiol Plant. 2008 Jul;133(3):481-9 PMID: 18346071
  65. Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling.
    Plant Cell. 2003 Jan;15(1):63-78 PMID: 12509522
  66. Maize ABI4 binds coupling element1 in abscisic acid and sugar response genes.
    Plant Cell. 2002 Oct;14(10):2565-75 PMID: 12368505
  67. Targets of the WRKY53 transcription factor and its role during leaf senescence in Arabidopsis.
    Plant Mol Biol. 2004 Aug;55(6):853-67 PMID: 15604721
  68. Targets of AtWRKY6 regulation during plant senescence and pathogen defense.
    Genes Dev. 2002 May 1;16(9):1139-49 PMID: 12000796
  69. Hv-WRKY38: a new transcription factor involved in cold- and drought-response in barley.
    Plant Mol Biol. 2004 May;55(3):399-416 PMID: 15604689
  70. Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression.
    Plant Cell. 2001 Jul;13(7):1527-40 PMID: 11449049
  71. Characterization of the expression of a desiccation-responsive rd29 gene of Arabidopsis thaliana and analysis of its promoter in transgenic plants.
    Mol Gen Genet. 1993 Jan;236(2-3):331-40 PMID: 8437577
  72. Enhanced heat and drought tolerance in transgenic rice seedlings overexpressing OsWRKY11 under the control of HSP101 promoter.
    Plant Cell Rep. 2009 Jan;28(1):21-30 PMID: 18818929
  73. Four tightly linked rab genes are differentially expressed in rice.
    Plant Mol Biol. 1990 Jan;14(1):29-39 PMID: 2151651
  74. Mutations in ABO1/ELO2, a subunit of holo-Elongator, increase abscisic acid sensitivity and drought tolerance in Arabidopsis thaliana.
    Mol Cell Biol. 2006 Sep;26(18):6902-12 PMID: 16943431
  75. Overexpression of an R1R2R3 MYB gene, OsMYB3R-2, increases tolerance to freezing, drought, and salt stress in transgenic Arabidopsis.
    Plant Physiol. 2007 Apr;143(4):1739-51 PMID: 17293435
Article Info
Journal
The Plant journal : for cell and molecular biology
Abbr.
Plant J
ISSN
1365-313X
DOI
10.1111/j.1365-313X.2010.04248.x
Published
2010-08-00
Epub
2010-00-06
Pages
417-29
Language
English
Region
England
NLM ID
9207397
PMCID
PMC3117930
Subset
IM
Grants
NIGMS NIH HHS · R01 GM059138 · United States
NIGMS NIH HHS · R01 GM059138-15 · United States
External Links
PubMed source DOI: 10.1111/j.1365-313X.2010.04248.x PMC Full Text View journal details
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