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PMID: 9634591 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The Arabidopsis abscisic acid response locus ABI4 encodes an APETALA 2 domain protein.

The Plant cell ·Vol. 10 ·No. 6 ·1998-06-00 ·Pages 1043-54

Finkelstein RR, Wang ML, Lynch TJ, Rao S, Goodman HM

Abstract

Arabidopsis abscisic acid (ABA)-insensitive abi4 mutants have pleiotropic defects in seed development, including decreased sensitivity to ABA inhibition of germination and altered seed-specific gene expression. This phenotype is consistent with a role for ABI4 in regulating seed responses to ABA and/or seed-specific signals. We isolated the ABI4 gene by positional cloning and confirmed its identity by complementation analysis. The predicted protein product shows homology to a plant-specific family of transcriptional regulators characterized by a conserved DNA binding domain, the APETALA 2 domain. The single mutant allele identified has a single base pair deletion, resulting in a frameshift that should disrupt the C-terminal half of the protein but leave the presumed DNA binding domain intact. Expression analyses showed that despite the seed-specific nature of the mutant phenotype, ABI4 expression is not seed specific.

MeSH Terms
Abscisic Acid/pharmacology Amino Acid Sequence Arabidopsis/drug effects,genetics,metabolism Arabidopsis Proteins Base Sequence Chromosome Mapping Gamma Rays Gene Expression Regulation, Plant/drug effects,physiology Genes, Plant Genetic Complementation Test Homeodomain Proteins/biosynthesis,chemistry,genetics Molecular Sequence Data Mutagenesis Nuclear Proteins/biosynthesis,chemistry,genetics Plant Proteins/biosynthesis Plants, Genetically Modified Polymorphism, Restriction Fragment Length Recombination, Genetic Seeds/physiology Sequence Alignment Sequence Homology, Amino Acid
Chemicals
APETALA2 protein, Arabidopsis Arabidopsis Proteins Homeodomain Proteins Nuclear Proteins Plant Proteins Abscisic Acid
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Finkelstein R R
Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, California 93106, USA. finkelst@lifesci.lscf.ucsb.edu
Wang M L
Lynch T J
Rao S
Goodman H M
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.10.6.1043
Email
finkelst@lifesci.lscf.ucsb.edu
Published
1998-06-00
Pages
1043-54
Language
English
Region
England
NLM ID
9208688
PMCID
PMC144030
Subset
IM
Databases
GENBANK
AF040959
External Links
PubMed source DOI: 10.1105/tpc.10.6.1043 PMC Full Text View journal details
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